不同发育阶段银杏胚的组织培养研究  

Study on Tissue Culture of Ginkgo Embryos from Different Developing Stages

作　　者：秦小舒[1] 逯岩[1] 郁万文[1] 曹福亮[1] 

机构地区：[1]南京林业大学森林资源与环境学院,江苏南京210037

出　　处：《北方园艺》2014年第23期87-90,共4页Northern Horticulture

基　　金：江苏省自然科学基金资助项目(BK2010019);国家自然科学基金资助项目(31170627)

摘　　要：以低温贮藏银杏胚为试材,研究不同取材时期银杏胚的生长长度变化、愈伤组织诱导和分化能力。结果表明:早期取材且低温保存,能减缓胚的生长和发育速度;不同时期胚均能较好的诱导愈伤组织,以10月10日取材在MS培养基上诱导率最高;愈伤组织有一定的分化能力,早期(10月3日至11月16日)取材能诱导出胚性愈伤和不定芽,晚期(11月16日以后)取材能诱导出不定芽和不定根,诱导频率较低。Taking cryopreservation Ginkgo embryos as materials to study embryos changes, callus induclion and differentiation during different stages. The results showed that, collecting immature embryo and cryopreservation could slow down the growth and development of embryos; embryo of different stages could derive callus well, materials from October 10th induced the highest rate of callus with MS medium; callus had the ability of differentiation： immature embryo （October 3rd to November 16th） could induce embryonic callus and adventitious buds, later （after November 16th） drawn to induce adventitious buds and adventitious roots. However,the frequency of callus differentiation was low.

关 键 词：银杏胚 不同时期 愈伤组织 

分 类 号：S666.903.6[农业科学—果树学]