生防枯草芽孢杆菌高效抗逆菌株选育及其对一品红灰霉病的防控效果  

Mutagenesis of Bacillus subtilis with High Toxicity and Strong Resilience,and Its Effect of Control of Botrytis cinerea of ‘Poinsettia'

作　　者：李娜[1] 刘锦霞[1] 杜文静[1] 李晶[1] 丁品[1] 张建军[1] 武建荣[1] 

机构地区：[1]甘肃省科学院生物研究所,甘肃兰州730000

出　　处：《北方园艺》2014年第23期101-105,共5页Northern Horticulture

基　　金：全国科学院联盟合作研究资助项目;陇原青年创新人才扶持计划资助项目

摘　　要：采用UV和NTG复合诱变的方法对原始生防枯草芽孢杆菌Bs-a进行了诱变选育;同时结合抗逆能力和室内外抑菌活性测定,以期筛选出抑菌活性和抗逆性均较强的优良灰霉菌生防菌株。结果表明：通过UV和NTG复合诱变的方法获得了枯草芽孢杆菌Bs-a的突变菌株D17和W03,2种菌株具有传代稳定、高毒力、强抗逆的特点,其抗紫外线能力极强,经过2.0h的紫外照射活菌浓度仍可达10^8 cfu/mL以上;可耐受的温度和pH值范围分别为0-60℃、pH 3-9,温室内对一品红灰霉病的相对防效可达85%以上,与出发菌Bs-a之间存在极显著差异,是2株具备产业化开发价值的灰霉病生防菌株。Taking the mutagenesis of Bacillus subtilis Bs-a by UV and NTG combined with the test of resilience and indoor and outdoor antibacterial activity, filter out two superior strains of bacteria D17 and W03, which had stable inheritance high toxicity,strong resilience. The results showed that the anti-UV ability of the bacterias were strong, the living bacteria concentration of which remained viable up to 10^8 cfu/mL or more after ultra-vioket radiation for 2 hours. The range of withstand temperature and pH were 0--60℃ and pH 3- 9, Their control effect of Botrytis cinerea of ＂Poinsettia＂ were more than 85~. It was significantly different with original strain Bs-a. D17 and W03 were industrial development valuable in microbial biocontrol bacteria.

关 键 词：枯草芽孢杆菌 高毒力 抗逆性 菌种选育 灰霉病 一品红 

分 类 号：S685.23[农业科学—观赏园艺] S476[农业科学—园艺学]