鬼鲉肝脏提取物对小鼠免疫功能的影响  

Influence of Inimicusjaponicasliver extract on mice's immune function

作　　者：葛雅辉 刘欣[1] 唐于平[1] 郭建明[1] 丁少雄[2] 林祥志[3] 林汝榕[3] 段金廒[1] 

机构地区：[1]南京中医药大学江苏省中药资源产业化过程协同创新中心和中药资源产业化与方剂创新药物国家地方联合工程研究中心,江苏南京210023 [2]厦门大学海洋与地球学院,福建厦门361005 [3]国家海洋局第三海洋研究所,福建厦门361005

出　　处：《中国海洋药物》2014年第6期32-38,共7页Chinese Journal of Marine Drugs

基　　金：海洋公益性行业科研专项(201205024-1)资助

摘　　要：目的研究鬼鲉肝脏提取物对免疫低下小鼠免疫功能的影响。方法采用环磷酰胺诱导建立免疫低下动物模型,免疫器官重量法测定胸腺指数和脾脏指数,碳粒廓清法测定小鼠单核巨噬细胞的功能,绵羊红细胞免疫法测定小鼠体液免疫功能,采用血细胞计数仪检测小鼠外周血常规,采用ELISA方法测定小鼠血清中TNF-α、IL-1β、IL-2、IL-6的水平,采用流式细胞仪测定脾细胞周期。结果鬼鲉肝脏提取物可以减缓免疫低下小鼠体重的下降趋势以及脾脏和胸腺的损伤、促进其脾细胞的分裂和增殖、增加其单核巨噬细胞吞噬功能和血清溶血素含量以及白细胞数、中性粒细胞数、淋巴细胞数和单核细胞数和血清中TNF-α、IL-1β、IL-2、IL-6的含量。结论鬼鲉肝脏提取物可以增强免疫低下小鼠的免疫功能。Objective To investigate the influence of Inimicusjaponicas liver extract on the immune function of immunosuppressed mice. Methods Irnmunosuppression animal model was established by cyclo- phosphamide induction. The immunity organs were weighed to measure the thymus index and spleen index; the clearance of charcoal particles method was used to measure mice＇s mononuclear macrophages functions, and sheep red blood cells immunizations was used to measure mice＇s humoral immune func tions; mice＇s blood counts were measured by blood-counter system, and Enzyme-linked Immuno Sor- bent Assay （ELISA） was used to measured the TNF-α, IL-1β, IL-2 and IL-6 level in blood serum; the spleen cell cycle was measured by flow cytometry. Results Inirnicus japonzcas ttver extract slgmcmtly improved the thymus index and spleen index, promoted the division and proliferation of spleen cells, improved the mononuclear macrophages functions, increased the hemolysin level of blood serum and the levels of leukocytes, neutrophils, lymphocytes and monocytes, and also increased the levels of TNF-α, IL-1β, IL-2 and IL-6 in blood serum. Conclusion Inimicus japonicas liver extract enhanced immunosup pressed mices immune function.

关 键 词：鬼鲉 肝脏提取物 体液免疫 细胞免疫 细胞因子 

分 类 号：R965[医药卫生—药理学]