HPLC法测定复方菝葜颗粒中落新妇苷的含量  

Determination of astilbin in compound Baqia granules by high-performance liquid chromatography

作　　者：陈新元 谢谊[2] 刘玉琴[2,3] 王实强[2] 

机构地区：[1]湖南国华制药有限公司,湖南长沙410013 [2]湖南省中医药研究院中药研究所,湖南长沙410013 [3]湖南中医药大学,湖南长沙410208

出　　处：《湖南中医杂志》2014年第11期163-165,共3页Hunan Journal of Traditional Chinese Medicine

摘　　要：目的：建立高效液相法测定复方菝葜颗粒中落新妇苷含量的测定方法。方法：采用Waters SymmetryC18（150×4.6mm,5μm）为色谱柱,流动相为甲醇-0.3%冰醋酸（32∶68）,流速为1.0m L/min;检测波长为291nm。结果：落新妇苷的线性范围为0.12~1.2μg,平均回收率为98.19%,RSD为0.82%。结论：所建立方法操作简便、准确、可靠、重复性好,可用于复方菝葜颗粒中落新妇苷的含量测定。Objective： To establish a method for determining the content of astilbin in Baqia granules by high-performance liquid chromatography（ HPLC）. HPLC was performed on a Waters Symmetry C18column（ 150 ×4. 6mm,5μm） with a mobile phase of methanol-0. 3% glacial acetic acid（ 32∶ 68） at a flow rate of 1. 0ml / min and a detection wavelength of 291 nm. Results： A linear relationship was found within an astilbin content range of0. 12-1. 2μg. The average recovery rate was 98. 19%,and the relative standard deviation was0. 82%. Conclusion： This HPLC method is simple,accurate,reliable,and repeatable and can be used for the determination of astilbin in compound Baqia granules.

关 键 词：高效液相色谱法 复方菝葜颗粒 落新妇苷 含量测定 

分 类 号：R284.1[医药卫生—中药学]