环卵沉淀试验在广州管圆线虫病诊断中的应用  

Diagnosis of Angiostrongyliasis cantonensis by circum oval precipitin test

作　　者：薛士鹏[1] 

机构地区：[1]南阳医学高等专科学校科研实验中心,河南南阳473061

出　　处：《中国热带医学》2014年第11期1358-1360,共3页China Tropical Medicine

摘　　要：目的评价环卵沉淀试验(Circum oval precipitin test COPT)在广州管圆线虫病检测诊断中的应用价值。方法根据广州管圆线虫感染时间不同将SD大鼠分为B、C、D 3组,(A组为正常SD大鼠,E、F组分别为感染血吸虫、旋毛虫的SD大鼠),每组5张单凹载玻片,每张采用10个广州管圆线虫虫卵,与感染前后不同时间段大鼠血清各30μL进行反应,放置370C孵箱内孵育,72h后置显微镜下观察阳性反应结果并计算环沉率。结果广州管圆线虫幼虫感染后7d、14d和28d,SD大鼠血清环沉率分别为24%、68%、90%;感染前SD大鼠血清、血吸虫阳性血清和旋毛虫阳性血清广州管圆线虫环沉率分别为0%、0%和2%。结论 COPT法检测感染广州管圆线虫病的大鼠血清抗体具有一定的免疫学诊断价值,为广州管圆线虫病散发病例的快速诊断提供可选择的方法。Objective To evaluate the value of circum oval precipitin test in diagnosis of Angiostrongyliasis cantonensis.Methods According to the different time of infection, Angiostrongylus cantonensis speciments were marked as group B, Cand D. While serum from normal rats was marked as group A and positive serums from rats infected with Schistosome andTriehinella spiralis were marked as group E and F respectively. Each group composed of 5 concave slides coated with 10 eggsof Angiostrongyliasis cantonensis and reacted with30μL serum from rats after infected with Angiostrongyliasis cantonensis atvarious length of duation. Then the slides were incubated in hatch box 37℃ for 72h and the results were analyzed undermicroscope. Results After infection of Angiostrongylu cantonensis for 7d 14d and 28d, the positive rates of SD rat serumwere 24%, 68% and 90% respectively. While the positive rates of normal SD rats serum, the positive serua of Schistosomiasisor Trichinella were 0, 0 and 2%,respectively. Conclusion Circum oval preeipitin test was useful in immunological diagnosisof sporadic Angiostrongyliasis cantonensis and it can be used for rapid detection of Angiostrongyliasis eantonensis inGuangzhou.

关 键 词：广州管圆线虫 环卵沉淀试验 环沉率 

分 类 号：R532[医药卫生—内科学]