活血潜阳颗粒对SHR大鼠尾动脉血压、24h尿总蛋白、肾小球TGF-β1、α-SMA作用的免疫组化学定量分析  

Immunohistochemical quantitative research on the effect of Huoxue Qianyang Capsule on SHR rats arterial blood pressure,24 h urinary total protein,glomerular TGF-β1 and α-SMA

作　　者：唐补生 张伟珍[2] 曹敏[2] 周端[2] 

机构地区：[1]上海市奉贤区中医医院,上海201400 [2]上海中医药大学附属龙华医院,上海200032

出　　处：《长春中医药大学学报》2014年第6期971-974,共4页Journal of Changchun University of Chinese Medicine

基　　金：上海市中医临床重点学科建设项目(ZYXK2012007)

摘　　要：目的研究活血潜阳颗粒对SHR大鼠血压、24 h尿蛋白定量、肾小球TGF-β1、α-SMA免疫组化学定量分析,探讨活血潜阳法治疗高血压肾病的可能机制,为活血潜阳颗粒的肾靶器官保护的临床运用提供实验依据。方法 42只SPF级19周龄雄性SHR大鼠根据体质量随机分为活血潜阳颗粒大、中、小剂量组,糜酶抑制剂组和模型组;8只SPF级19周龄WKY大鼠作为正常对照组。活血潜阳颗粒大、中、小剂量组分别予活血潜阳颗粒清膏灌胃;糜酶抑制剂组给予SBTI 10 mg/(kg·d)灌胃,模型组和WKY组双蒸水5 m L/(d·只)灌胃;连续4周。检测尾动脉血压、24 h尿总蛋白、肾小球TGF-β1和α-平滑肌肌动蛋白的免疫组化学表达。结果大、中剂量组TGF-β1阳性表达Area低于模型组,高于WKY组,差异均有统计学意义(P<0.05),与糜酶抑制剂组比较,P>0.05;小剂量组TGF-β1阳性表达Area低于模型组,P<0.05,大剂量组TGF-β1阳性表达IOD与模型组和糜酶抑制剂组比较,P<0.05,与WKY组比较,P>0.05,中、小剂量组TGF-β1阳性表达IOD与模型组、WKY组和糜酶抑制剂组比较,P<0.05,活血潜阳颗粒各组TGF-β1阳性表达IOD比较,P>0.05;大、中、小剂量组α-SMA阳性表达Area与模型组比较,P<0.05,中剂量组和WKY组比较,P<0.05,其余均P>0.05;大、中、小剂量组α-SMA阳性表达IOD与模型组比较,P<0.05,中剂量组α-SMA阳性表达IOD与WKY组比较有差异,大、小剂量组α-SMA阳性表达IOD与wky组比较无统计学意义,活血潜阳颗粒各组之间α-SMA阳性表达IOD比较,P>0.05。结论活血潜阳颗粒可能通过降低血压及减少24 h尿蛋白排出,从而抑制肾小球TGF-β1、α-SMA的免疫组学表达,延缓肾纤维化,其中中等剂量可能是最佳剂量选择。Objective Through the immunohistochemical quantitative research on the effect of Huoxue Qianyang Capsule on SHR rats arterial blood pressure, 24 h urinary total protein, glomerular TGF-β1 and α-SMA, the possible mechanism of treat- ing hypertensive nephropathy by the method of Huoxue Qianyang, thus laying the experimental basis for clinical applications of Huoxue Qianyang Capsule in protecting target organs like the kidney. Methods 42 nineteen-weeks male SHR rats ot SPF grade were randomly grouped into large dose group,middle dose group and small dose group of Huoxue Qianyang Cap- sule, chymase enzyme inhibition group, and model control group according to weight; 8 nineteen-weeks male SHR rats of SPF grade were normal control group. The large dose group, middle dose group and small dose group of Huoxue Qianyang Capsule were given Huoxue Qianyang Capsule cream for intragastic administration; chymase enzyme inhibition group were given 10 mg（kg· d） SBTI for intragastic administration;model control group and WKY group were given 5 mL/d double distilled water for intragastic administration for the consecutive 4 weeks. And then immunohistochemical expressions of arterial blood pres- sure,24 h urinary total protein, glomerular TGF-β1 and α-SMA were detected. Results The positive expression Area of TGF-β1 in middle and large dose groups was lower than that in model control group but higher than that in WKY group,the comparative difference had statistical significance（ P 〈 0.05）. When compared with cllymase enzyme inhibition group, P 〉 0.05;the positive expression Area of TGF-β1 in small dose group was lower than that in the model control group（ P 〈 0.05） ;when the positive expression IOD of TGF-β1 was compared with the model control group and the chymase enzyme in- hibition group, P 〈 0. 05, when the positive expression IOD of TGF-β1 was compared with the WKY group, P 〉 0.05. When the positive expression IOD of TGF-β1 of the middle and small dose group was compared with the model control g

关 键 词：高血压肾纤维化 活血潜阳颗粒 TGF-Β1 Α-SMA 

分 类 号：R285.5[医药卫生—中药学]