胃癌细胞MKN-45和SGC-7901中MTA1的泛素化修饰及其对MTA1表达的影响  

Ubiquitination and expression of MTA1 in gastric cancer MKN-45 and SGC-7901 cells

作　　者：付海京[1,2] 王锐[1,2] 陈一天[1,2] 张群[1,2] 孙茜[1,2] 黄小银[1,2] 管晓翔[1,2] 王靖华[1,2] 陈龙邦[1,2] 

机构地区：[1]南京大学医学院临床学院 [2]南京军区南京总医院(金陵医院)肿瘤内科,江苏南京210002

出　　处：《南京医科大学学报（自然科学版）》2014年第11期1503-1506,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金(81272734;81372588)

摘　　要：目的 :转移相关基因1(metastasis associated 1,MTA1)已知与肿瘤转移密切相关,且与胃癌恶性程度明显相关,并可以通过影响信号转导途径发挥其功能,本研究拟确定MTA1是否可以被泛素化途径所调控。方法:以蛋白酶体抑制剂MG-132处理胃癌细胞后,以IP/WB实验检测MTA1的表达水平,并将带Myc标签的MTA1质粒以及带HA标签的泛素(HA-Ub)质粒转入该细胞,检测Myc-MTA1的表达。结果:以蛋白酶体抑制剂MG-132处理胃癌细胞MKN-45、SGC-7901后,MTA1表达水平显著增加;免疫共沉淀实验显示泛素分子可以与MTA1直接结合,并且实验组较对照组相比泛素化程度明显增加;将HEK293细胞以si MTA1处理封闭MTA1的表达后,将Myc-MTA1质粒以及HA-Ub质粒转入该细胞,发现当后者存在时Myc-MTA1可被强烈泛素化。结论:在胃癌细胞中MTA1是可被泛素化途径修饰的蛋白。Objective：Metastasis associated 1（MTA1）is a protein closely related to the malignant degree of gastric cancer. We try to prove that MTA1 can be regulated by the ubiquitin pathway in this study. Methods：After the proteasome inhibitor MG-132 treatment in gastric cancer cells,the expression level of MTA1 was detected though IP / WB. MTA1 expressing plasmid containing the Myc tag and HAtagged ubiquitin plasmid（HA-Ub） were co-transfected into cells,and the expression of Myc-MTA1 was detected.Results：After treatment with MG-132,the protein level of MTA1 in gastric carcinoma cell line SGC-7901 increased significantly.Myc-MTA1 was strongly ubiquitinated by HA-Ub in HEK293 cells. Conclusion：MTA1 in human gastric cancer cells can be regulated through ubiquitination pathway.

关 键 词：胃癌 转移相关基因1 泛素化 

分 类 号：R735.2[医药卫生—肿瘤]