西芹根物质四次酮层物对黄瓜枯萎病菌细胞壁降解酶活性及镰刀菌酸含量的影响  

Effects of acetone chromatography extracts of parsley root materials on the cell wall degrading enzymes activities and fusaric acid contents of Fusarium oxysporum f. sp. cucumerinum

作　　者：高晓敏[1] 马立国[1] 郝静[1] 潘静[1] 李杰[1] 云兴福[1] 

机构地区：[1]内蒙古农业大学农学院,呼和浩特010019

出　　处：《生态学杂志》2014年第12期3388-3394,共7页Chinese Journal of Ecology

基　　金：国家自然科学基金项目(31160100);内蒙古自治区应用技术研究与开发项目(20110711)资助

摘　　要：为了探讨西芹根物质中化感物质对黄瓜枯萎病菌生理生化的作用,本研究对西芹根物质丙酮浸提液进行4次柱层析,每次层析后均进行化感效果测定,以化感抑制效果筛选每次层析最佳流分;然后将最佳流分和黄瓜枯萎病菌在液体培养基条件下进行共培养,144 h后测定黄瓜枯萎病菌分泌的3种细胞壁降解酶活性以及镰刀菌酸的含量。结果表明:西芹鲜根及根际土丙酮浸提液4次层析物每次层析获得的最佳流分均能抑制黄瓜枯萎病菌的生长;随层析次数的增加细胞壁降解酶活性和镰刀菌酸含量呈下降趋势,且细胞壁降解酶活性和镰刀菌酸含量较对照差异显著;相关性分析结果显示,各层析物化感抑制效果与果胶酶和β-葡萄糖苷酶的活性呈显著负相关,与镰刀菌酸含量无显著相关关系。The acetone extracts of parsley root materials obtained by four times column chromatography were used for exploring the effects of allelochemicals in the parsley root materials on physiological and biochemical aspects of Fusarium oxysporum f. sp. cucumebrium. Best column fractions were screened by the allelopathic inhibition effect and the activities of three cell wall degrading enzymes and fusaric acid content after 144 h of co-culture with F. oxysporum f. sp. cucumebrium in the liquid medium. The results indicated that the best column fractions obtained from the acetone extracts of fresh parsley root and rhizosphere soil inhibited the growth of F. oxysporum f. sp. cucumebrium. In comparison to CK,the cell wall degrading enzymes activities and fusaric acid contents decreased significantly with increased numbers of chromatography. The correlation analysis indicated that allelopathic inhibition effect had significant negative correlations with the activities of pectinase and β-glycosidase enzymes,but no significant correlation with the fusaric acid content.

关 键 词：西芹根物质 酮层物 细胞壁降解酶 镰刀菌酸 黄瓜枯萎病菌 

分 类 号：S436.421.13[农业科学—农业昆虫与害虫防治]