压力灌注对不同程度积水肾线粒体损伤的影响  

Effects of mitochondrial damage in different levels of hydronephrosis under pressure perfusion

作　　者：余伟民[1] 姚小兵[1] 曹智修[1] 饶婷[1] 李维[1] 程帆[1] 

机构地区：[1]武汉大学人民医院泌尿外科,430060

出　　处：《中华实验外科杂志》2014年第12期2688-2689,共2页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81200501）

摘　　要：目的 观察急性肾盂内压升高对不同程度积水肾线粒体损伤的影响.方法 采用腰大肌包埋法建立新西兰大白兔轻度和重度肾积水模型,轻度积水组分为M0、M1、M2和M3组,重度积水组分为S0、S1、S2和S3组,对应分别以0、20、60、100 mmHg（1 mmHg =0.133 kPa）压力进行肾盂灌注,观察肾组织标本线粒体超微结构改变和线粒体膜电位的变化.结果 轻度积水组经100 mmHg灌注后（M3）,线粒体空泡化比例为（66.33 ±4.42）％,JC-1多聚体/单体荧光强度（Q2/Q4）的值为0.36±0.07,和对照组（M0）比较差异有统计学意义（P＜0.05）,但M1和M2组变化不明显（P＞0.05）;与对照组（S0）比较,重度积水组经60、100 mmHg灌注后（S2和S3）,线粒体空泡化比例分别为（85.67±8.61）％和（87.50±5.77）％,Q2/Q4的值分别为0.42±0.07和0.23±0.04,差异有统计学意义（P＜0.05）,但S1与SO比较差异无统计学意义（P＞0.05）.结论 肾盂灌注压力的升高会导致线粒体空泡化和膜电位降低,不同程度积水肾对肾盂内压升高的耐受能力存在差异.Objective To investigate the mitochondrial damage of acute increased renal pelvis pressure to varying degrees of hydronephrosis.Methods The mild and severe hydronephrosis model in New Zealand white rabbits were established by psoas Embedding.Mild hydronephrosis group was divided into M0,M1,M2 and M3 group,severe hydronephrosis group was divided into S0,S1,S2 and S3 groups （n =6）,corresponding to 0,20,60 and 100 mmHg （1 mmHg =0.133 kPa） renal pelvis perfusion pressure,respectively.Specimens were detected after 48 h,including ultrastructural alterations observed and the mitochondrial membrane potential detected.Results The group of 100 mmHg perfusion （M3） in mild hydronephrosis,vacuolization of mitochondria ratio was （66.33 ± 4.42） % and the value of JC-1 polymer/ monomer fluorescence intensity （Q2/Q4） was 0.36 ± 0.07,which were significantly alter when compared to control group （M0,P 〈 0.05）,but no significant changes in M1 and M2 group （P 〉 0.05）.Compared with the control group （S0）,severe hydronephrosis group by 60 and 100 mmHg perfusion （S2 and S3）,vacuolization of mitochondria ratio were （85.67 ± 8.61） % and （87.50 ± 5.77） %,while the Q2/Q4 were 0.42 ± 0.07 and 0.23 ± 0.04,which were significantly changed （P 〈 0.05）,but no significant change in the S1 group.Conclusion Pelvis perfusion pressure rise will cause mitochondrial vacuolization and membrane potential decreases,the tolerance was differences in varying degrees of hydronephrosis when the renal pelvis perfusion pressure increased.

关 键 词：肾积水 线粒体损伤 线粒体超微结构 线粒体膜电位 

分 类 号：R692.2[医药卫生—泌尿科学]