原发性高血压大鼠阴茎海绵体组织中电导型钙激活性钾通道的表达  

The expression and correlation of intermediate conductance calcium-activated potassium channels in cavernous tissue of spontaneously hypertensive rats

作　　者：廖胜[1] 高飞[1] 葛余正[1] 周六化[1] 吴然[1] 贾瑞鹏[1] 

机构地区：[1]南京医科大学附属南京医院南京市第-医院泌尿外科,210006

出　　处：《中华实验外科杂志》2014年第12期2709-2711,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81070597、81370853）;江苏省卫生厅科教兴卫丁程领军人才与创新团队科研课题（LJ3201107）;江苏省六大人才高峰资助项目（2011-WS-093）;江苏省科教兴卫工程重点学科开放课题（KF200942）

摘　　要：目的 检测内皮依赖性超级化因子（EDHF）通路关键性物质中电导型钙激活性钾通道（IKCa）在原发性高血压大鼠阴茎海绵体组织的表达,探讨高血压对大鼠阴茎海绵体中IKCa表达的影响.方法 健康雄性原发性高血压大鼠（SHR） 20只及同系正常（WKY）大鼠10只,无创血压仪测量大鼠尾部收缩压（SBP）,颈部皮下注射阿扑吗啡（APO）检验大鼠阴茎勃起,将大鼠分为高血压勃起功能障碍组（HBP-ED）、高血压组（HBP）和对照组（Control）.运用Western blot和逆转录-聚合酶链反应（RT-PCR）技术检验大鼠阴茎海绵体中IKCa蛋白和mRNA的表达.结果 SHR组收缩压为（202.43±9.89）mmHg（1 mmHg =0.133 kPa）,与WKY组[（127.21±3.48） mmHg]比较差异有统计学意义（P＜0.01）,Western blot和RT-PCR显示IKCa蛋白及mRNA在WKY、HBP及HBP-ED组中的表达均呈递减变化,其中HBP-ED组显著低于WKY组（P＜0.01）及HBP组（P＜0.01）,WKY组与HBP组间比较差异无统计学意义（P＞0.05）.结论 高血压性勃起功能障碍可能与IKCa在原发性高血压大鼠阴茎海绵体中的表达低下密切相关.Objective Intermediate conductance calcium-activated potassium channels （IKCa）,one of the key material of the endothelium-derived hyperpolarizing factor （EDHF） passway.To detect the expression of IKCa and explore hypertension impact IKCa expression in cavernous tissue of spontaneously hypertensive rats.Methods Male spontaneously hypertensive rats （SHR） （n =20） and normotensive Wistar Kyoto rats （WKY,n =10） were studied,Systolic blood pressure （SBP） was measured by noninvasive blood pressure instrument and erectile function was tested by injecting with apomorphine （APO）,the rats were divided into 3 groups,namely hypertensive with erectile dysfunction （HBP-ED）group （n =13）,hypertensive （HBP） group （n =7） and control group （n =10）.The expression of IKCa gene mRNA and protein was assessed by Western blotting and reverse transcription-polymerase chain reaction （RT-PCR）.Results Versus the WKY group,SHR group showed a higher systolic blood pressure [（202.43 ±9.89）vs.（127.21 ± 3.48） mmHg （1 mmHg =0.133 k Pa）,P 〈 0.01].The expressions of IKCa protein and mRNA were the highest in the control group and the lowest in HBP-ED group.Conclusion The erectile function of penis is impacted seriously by hypertension,which may be related to the decreased expression of IKCa in cavernous tissue of spontaneously hypertensive rats.

关 键 词：高血压 阴茎勃起 阴茎海绵体 中电导钙激活性钾通道 

分 类 号：R544.11[医药卫生—心血管疾病]