京尼平交联对兔脱细胞气管基质生物力学性能及血管生成的影响  

Biomechanical and angiogenic properties of tissue-engineered rabbit trachea using genipin cross-linked decellularized tissue

作　　者：孙飞 潘枢 史宏灿 刘星辰 叶钢 张卫东 章方彪 张思泉 仲崇浩 袁晓龙 

机构地区：[1]扬州大学临床医学院胸外科扬州大学转化医学研究中心,225001

出　　处：《中华实验外科杂志》2014年第12期2764-2767,共4页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81370118、81170014）;江苏省“六大人才高峰”高层次人才基金资助项目（2009128）

摘　　要：目的 研究兔脱细胞组织工程气管支架的制备,进而行京尼平交联探讨其对脱细胞气管基质生物力学性能及血管生成性能的影响.方法 采用去污剂-联合酶法（DEM）对新西兰兔气管行脱细胞处理,对组织体系结构及机械性能评估,随后用京尼平交联,行生物力学测试及血管再生实验研究.结果 经7个周期DEM脱细胞处理能够获取保存有与原生组织相似的层次结构、机械性能及强烈的血管再生特性的组织工程脱细胞气管.DNA量化结果显示：脱细胞基质中DNA含量为（42.39±6.66） ng/mg,与原生组织的（586.78±53.56） μg/L比较,减少了92.78％ （P＜0.01）.与原生样本及脱细胞样本比较,1％京尼平交联气管基质弹性模量明显增加（P＜0.05）.与其他样本比较,经1％京尼平交联气管基质能够显著诱导血管生成.结论 7个周期DEM处理能够有效获取长段无免疫原性脱细胞兔组织工程气管.通过京尼平交联能够有效提高脱细胞气管机械性能,且不影响脱细胞气管基质血管生成性能,也不诱发体内炎性反应.Objective To investigate the preparation of the decellularized tracheal scaffold in rabbit,and investigate the impact of biomechanical and angiogenic properties of tissue-engineered rabbit trachea using genipin cross-linked decellularized tissue.Methods New Zealand rabbits tracheae were decellularized using a modified detergent-enzymatic treatment.Glycosaminoglycan content,histoarchitecture,and mechanical properties were evaluated.Then evaluate mechanical properties and in vivo pro-angiogenic properties of genipin-treated tracheal matrices.Results After 7 detergent-enzymatic cycles,almost complete decellularized tracheae,retaining the hierarchical and mechanical properties of the native tissues,could be obtained.DNA concentration in the tissue was substantially decreased 92.78% from （586.78 ± 53.56） ng/mg in native trachea to （42.39-±6.66） ng/mg after 7 cycles of decellularization （P 〈0.01）.A significant （P 〈 0.05） increase of the secant modulus for the cross-linked tracheae,compared to native and decellularized samples,was computed,especially in the case of samples treated with 1% geninin.In particular,the increase induced by tracheal matrices treated with genipin 1% resulted significantly different from the one induced by all the other samples.Conclusion In conclusion,this work suggests that 7 cycles of the modified detergent-enzymatic method （DEM） generates a bioengineered rabbit tracheal matrix that is structurally and mechanically similar to native trachea.The treatment with genipin increased mechanical properties,in term of secant modulus,without neither altering the pro-angiogenic properties of decellularized airway matrices or eliciting an in vivo inflammatory response.

关 键 词：组织工程 脱细胞 血管生成 机械性能 京尼平 

分 类 号：R318.08[医药卫生—生物医学工程]