富血小板血浆对光老化皮肤中胶原蛋白的影响  

The effect of platelet rich plasma on collagen in the photoaging skin

作　　者：童怡兰[1] 王松山[1] 李爱林[1] 谢亮[1] 熊菡萏 李倩倩[1] 郑梁[1] 

机构地区：[1]武汉大学人民医院整形外科,430060

出　　处：《中华实验外科杂志》2014年第12期2814-2816,共3页Chinese Journal of Experimental Surgery

摘　　要：目的 通过建立光老化皮肤模型,观察富血小板血浆（PRP）对光老化皮肤中的抗衰老作用.方法 SPF级大耳白兔12只,随机分为实验组和对照组.采用UVA灯照射颈部及背部裸露皮肤24 h的方法制备光老化模型,实验组用PRP注射到真皮浅层,对照组则为生理盐水注射.分别于注射前后取颈背部皮肤行常规苏木素-伊红（HE）染色,光镜下观察细胞;免疫组织化学法分析Ⅰ型胶原及Ⅲ型胶原.结果 对照组HE染色未见明显改变,实验组较前胶原纤维排列紧密,弹力纤维总面积增加,成熟胶原纤维明显增多,新生的胶原纤维减少,弹力纤维减少、纤细.对照组Ⅰ型胶原及Ⅲ型胶原未见明显改变,差异无统计学意义（P＞0.05）,实验组Ⅰ型胶原及Ⅲ型胶原可见明显改变,差异有统计学意义（P＜0.05）.结论 作为皮肤老化指标的Ⅰ型胶原及Ⅲ型胶原,在注射PRP后的皮肤中的表达可见明显改变.Objective Through the establishment of photoaging skin model,research the change in the photoaging skin model,and the Anti-aging effect of platelet rich plasma （PRP） in the photoaging skin.Methods Twelve healthy Big ear rabbits were randomly divided into two groups：experimental group and control group.After molding,take the back of the neck skin do conventional HE staining,observe cells from the light-microscopy; Then,the control group use the normal saline for hypodermic on the back of the neck skin,while the experimental group use platelet rich plasma （PRP）.Take the back of the neck skin to do conventional HE staining before and after injection,observe cells from the light-microscopy; Immunohistochemistry analysis the collagen.Results Under light-microscopy,control group did not change significantly.But in experimental group,collagen fibers packed tightly and the total area of elastic fibers increase d.Mature collagen fibers increased significantly with the nascent collagen fibers reduced; After injection of saline and platelet rich plasma （PRP）,The expressions of collagen Ⅰ and collagen Ⅲ did not change obviously （P 〉 0.05）,but in the experimental group,the expressions obviously changed （P 〈0.05）.Conclusion As the indicator of skin aging,the expressions of collagen Ⅰ and collagen Ⅲ changed significant after PRP injection.

关 键 词：富血小板血浆 光老化 Ⅰ型胶原 Ⅲ型胶原 

分 类 号：R622[医药卫生—整形外科]