应用自制装置建立微侵袭小鼠急性脊髓损伤模型  

Establishment of minimally acute spinal cord injury in mice model by the self-made device

作　　者：董洲[1] 张可可[1] 陈学周[1] 李光宇[1] 方望[1] 尤涛[2] 汤健[1] 

机构地区：[1]安徽医科大学第一附属医院骨外科,合肥230032 [2]武汉大学人民医院

出　　处：《中华实验外科杂志》2014年第12期2916-2917,共2页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81171797）;湖北省自然科学基金资助项目（2011CHB022）

摘　　要：目的 构建自制的脊髓打击仪以及简易棘突间的牵开及稳定装置,探讨利用其建立保留椎板微侵袭小鼠急性脊髓损伤模型的可行性.方法 利用电动推杆、压力传感器等制备脊髓打击仪及棘突间的牵开稳定装置;将32只成年雌性KM小鼠随机分成4组,利用上述装置分别以无打击（0 g）、轻度（5 g）、中度（10 g）、重度（20 g）打击力建立保留椎板的小鼠脊髓损伤模型.术后12 h每组随机取2只小鼠行脊髓损伤区苏木素-伊红（HE）染色病理切片,并在术后1、3、7、14d行小鼠后肢运动评分（BBB评分）.结果 成功制造了脊髓打击仪及简易棘突间牵开稳定装置,并建立了保留椎板的微侵袭小鼠脊髓损伤模型;脊髓损伤的病理学检查结果及后肢运动评分在不同打击力度下差异有统计学意义（P＜0.05）.结论 应用自制的装置可建立稳定、可靠的保留椎板的微侵袭小鼠脊髓损伤模型,有助于脊髓损伤研究的进一步开展.Objective Try to construct the self-made spinal impactor and the simple interspinous process distraction stabilizing device.Discussion on the feasibility,stability and reliability of method establishing the acute spinal cord injury in mice model retaining the lamina.Methods Using a electric push fool,a pressure sensor and so on,to manufacture the spinal inpactor and the interspinous process distraction stabilizing device.32 adult female rats were randomly divided into four groups,using the self-made spinal impactor device to make varying degrees on the spinal cord of the mice model retaining the lamina,non-combat （0 g）,mild （5 g）,medium （10 g）,and severe （20 g）.Taking two mice from every group randomly and making pathological section of damage zone spinal cord after 12 h,testing the behavior BBB grade post operation 1,3 d,7,14 w,in order to evaluate its correlation with the strike force.Results The spinal impactor device and the interspinous process distraction stabilizing device was producted successfully,and the minimally invasive spinal cord injury model retaining the lamina was built.Pathological examination and behavioral score was related with the strike force closely and the grade differences was obvious （P 〈 0.05）.Conclusion A a stable and reliable spinal cord injury model retaining the lamina can be made by the self-made device,and this conducive to the further research on spinal cord injury.

关 键 词：自制装置 微侵袭 脊髓损伤 小鼠模型 

分 类 号：R651.2[医药卫生—外科学]