HPLC法测定白花蛇舌草中槲皮素-3-O-β-d-吡喃葡萄糖苷的含量  

Content Determination of Quercetin-3-O-β-d-glucopyranoside in Hedyotis diffusa by HPLC

作　　者：曹广尚[1] 杨培民[1] 周鹏[1] 李芳[1] 

机构地区：[1]山东中医药大学附属医院,济南250011

出　　处：《中国药房》2014年第47期4453-4454,共2页China Pharmacy

基　　金：国家自然科学基金资助项目(No.81274052);山东省自然科学基金资助项目(No.ZR2011HL043)

摘　　要：目的：建立测定白花蛇舌草中槲皮素-3-O-β-d-吡喃葡萄糖苷含量的方法。方法：采用高效液相色谱法。色谱柱为Kromasil C18（250 mm×4.6 mm,5μm）,流动相为乙腈-1%冰醋酸（60∶40,V/V）,检测波长为254 nm,流速为1.0 ml/min,柱温为35℃。结果：槲皮素-3-O-β-d-吡喃葡萄糖苷的质量浓度在5.24～52.40μg/ml范围内与其峰面积积分值呈良好线性关系（r=0.999 9）;精密度、稳定性、重复性试验的RSD均〈1.97%;平均加样回收率为99.57%,RSD=1.28%（n=6）。结论：该方法操作简便,结果准确、可靠,可用于白花蛇舌草药材中黄酮类活性成分的含量测定。OBJECTIVE： To develop a method for the content determination of quercetin-3-O-β-d-glucopyranoside in Hedyotis diffusa. METHODS： HPLC method was adopted. The determination was performed on kromasil C18（250 mm×4.6 mm, 5 μm）col- umn with mobile phase consisted of acetonitrile-0.1% acetic acid （60：40, V/V） at the flow rate of 1.0 ml/min. The detection wave- length was set at 254 nm, and column temperature was 35 ℃. RESULTS： The linear range of quercetin-3-O-β-d-glucopyranoside were 5.24-52.40 μg/ml （r=0.999 9） with an average recovery of 99.57% （RSD=1.28% ,n=6） ; RSDs of precision, stability and reproducibility tests were all lower than 1.97%. CONCLUSIONS： The method is simple, accurate and reliable, and can be used for the quality control of H. diffusa.

关 键 词：白花蛇舌草 槲皮素-3-O-β-d-吡喃葡萄糖苷 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]