补肾活血方对大鼠血管内皮细胞p16基因表达的影响  

Effect of Bushen Huoxue Recipe on the Expression of p16 Gene of Vascular Endothelial Cells in Rats

作　　者：张毅 蔡智刚 唐新征 

机构地区：[1]广东省深圳市福田区中医院内科,518034

出　　处：《临床合理用药杂志》2014年第33期17-18,共2页Chinese Journal of Clinical Rational Drug Use

摘　　要：目的观察补肾活血方对大鼠血管内皮细胞p16基因表达的影响。方法将健康雄性SD大鼠24只随机分为中药组、黄芪组及0.9%氯化钠溶液组,每组8只,分别给予每组大鼠等量补肾活血方、黄芪、0.9%氯化钠溶液灌胃。提取含药血清培养人脐静脉内皮细胞,采用β-半乳糖苷酶(β-gal)染色鉴定细胞衰老;流式细胞术分析细胞周期变化,RT-PCR法检测p16基因的表达。结果中药组、黄芪组血清培养细胞与0.9%氯化钠溶液组比较,G0/G1期细胞比例均减少(P<0.05),且中药组G0/G1期细胞比例低于黄芪组(P<0.05)。中药组、黄芪组血清培养细胞p16基因表达较0.9%氯化钠溶液组下调(P<0.05),且中药组血清培养细胞p16基因的表达较黄芪组下调(P<0.05)。结论补肾活血方具有延缓大鼠血管内皮细胞衰老的作用,其分子作用机制可能为通过调控血管内皮细胞p16的表达而使细胞周期停滞于G1期。Objective To investigate the effect of Bushen Huoxue Recipe on the expression of p16 gene of vascular endothelial cells in rats. Methods A total of 24 healthy male SD rats were randomly divided into groups A,B,C,8 rats in each group. A group given Bushen Huoxue Recipe,B group given Astragalus Membranaceus,C group given 0. 9% sodium chloride solution,the dosage of each group was equal,and the drug-delivery way was gavage. Human umbilical vein endothelial cells were cultivated by medicated serum,β-galactosidase staining was used to authenticate cell senescence,flow cytometry was used to analyze the changes of cell cycle,and RT-PCR method was used for the detection of expression of p16 gene. Re-sults G0/G1 cell proportion of groups A and B was lower than that of C group,respectively,and that of A group was lower than that of B group（ P〈0. 05）. Expression of p16 gene of groups A and B was lower than that of C group,respectively,and that of A group was lower than that of B group（P〈0. 05）. Conclusion Bushen Huoxue Recipe can delay the senescence of vascular en-dothelial cells in rats,its molecular mechanism may be regulate expression of p16 gene to make cell cycle stagnated at G1 phase.

关 键 词：内皮细胞 基因 p16 补肾活血方 大鼠 Sprague—Dawley 

分 类 号：R285.5[医药卫生—中药学]