hVEGF基因修饰的组织工程化复合物修复牙周组织缺损的实验研究  

hVEGF Gene Modified Tissue-engineered Compound in the Reconstruction of Periodontal Tissue Defects in SD Rats

作　　者：赵俊杰[1] 朱小峰[2] 闫福华[1] 

机构地区：[1]南京大学口腔医学院,江苏省南京市210008 [2]福建医科大学附属第一医院,福建省福州市350004

出　　处：《组织工程与重建外科杂志》2014年第5期250-254,共5页Journal of Tissue Engineering and Reconstructive Surgery

基　　金：南京市国际合作项目(201303051);福建省教育厅资助项目(JK2010023)

摘　　要：目的探讨hVEGF165基因修饰的BMSCs在SD大鼠急性牙周缺损模型中对牙周组织再生的作用。方法人工构建SD大鼠急性牙周缺损模型。设以下6组(36只SD大鼠,n=6):空白对照(A组);单纯e-PTFE膜(B组);BME+e-PTFE膜(C组);BMSCs+BME+e-PTFE膜(D组);空转BMSCs+BME+e-PTFE膜(E组);hVEGF165基因修饰的BMSCs+BME+e-PTFE膜(F组)。分别将转染hVEGF165基因的BMSCs与未转染的BMSCs接种于胶原膜BME-10X后,按各分组植入人工牙周缺损内,并以空白胶原膜和空载体为对照。4周后取材作病理切片,HE染色观察牙周组织再生情况,测量新生牙槽骨面积、新生牙骨质面积以及新生牙周膜宽度,结果进行统计学分析。结果各组均可见不同程度的牙周组织再生,A组、B组和C组三组新生牙槽骨面积(NB)、新生牙骨质面积(NC)和新生牙周膜宽度(NP)均无明显差别(P>0.05);与A组相比,D组、E组、F组的NB、NC均有显著增加(P<0.05);与E组相比,转染有hVEGF165基因组(F组)的NB、NC和NP均有显著增加(P<0.05)。结论 hVEGF165基因转染BMSCs与胶原膜复合物可促进大鼠牙周组织再生。Objective To investigate the effects of hVEGF165 gene transfected bone marrow stem cells （BMSCs） in the repair of periodontal defect in rats. Methods A model of rat periodontal defect was established. The gene-modified tissue- engineered complex, constructed by transfected BMSCs and BME-10X, was implanted into subcutaneous sites in SD rats. The 36 SD rats were divided into 6 groups randomly （n：6）： control （A and B）, blank collagen membranes （C）, BMSCs with collagen membranes （D）, empty adenoviral vector transfected BMSCs with collagen membranes （E）, and hVEGF165 transfected BMSCs with collagen membranes （F）. BMSCs with different treatments were seeded on the collagen membranes. The collagen membrane with or without cells was transplanted into an acute periodontal defect model respectively, and then the defects were covered with e-PTFE membranes. Histological and morphometric analysis were performed 4 weeks after operation, the area of new alveolar bone （NB）, cementum area （NC） and the width of periodontal ligament （NP） were measured and compared. Results Different degrees of periodontal tissue regeneration were observed in all groups. There were no differences of the area of newly formed alveolar bone （NB）, cementum area （NC） and the width of periodontal ligament （NP） among the 3 groups （A, B, C）. NB and NC in group D, E, F were significantly increased compared with group A （P〈0.05）. NB, NC and NP in group F were significantly increased compared with group E （P〈0.05）. Conclusion The BME-IOXshowed a good biocompatibility with BMSCs transfected by hVEGF165. BMSCs transfected by hVEGF165 gene enhanced periodontal tissue regeneration in models of periodontal defect.

关 键 词：人血管内皮细胞 骨髓基质干细胞 组织工程 牙周组织再生 胶原膜 急性牙周缺损 

分 类 号：Q813.11[生物学—生物工程]