High-performance liquid chromatographic fingerprint analysis of Oxytropis falcata Bunge and Oxytropis chiliophylla Royle  

藏药镰形棘豆和轮叶棘豆的高效液相色谱指纹图谱分析(英文)

作　　者：格桑罗布[1,2] 确生[3] 格桑帕卓 张晓晶[1,2] 桑罗 梁鸿[1,2] 张庆英[1,2] 

机构地区：[1]北京大学医学部天然药物及仿生药物国家重点实验室 [2]天然药物学系,北京100191 [3]青海师范大学民族师范学院,青海西宁810008 [4]西藏自治区藏医药研究院,西藏拉萨85000

出　　处：《Journal of Chinese Pharmaceutical Sciences》2014年第11期783-789,共7页中国药学（英文版）

基　　金：National Natural Science Foundation of China (Grant No.21372015 and 20872006)

摘　　要：A simple, sensible and reliable HPLC-DAD fingerprint analysis method for the raw materials of Oxytropisfalcata and Oxytropis chiliophylla, both of which were used as ＂Er-Da-Xia＂ in Tibetan medicines, was developed and then subsequently applied to analyze samples collected from different locations or times. 19 common fingerprint peaks for O. falcata, 24 for O. chiliophylla, and 11 for the two herbs were designated respectively, including 7 identified characteristic peaks existing in both herbs and 1 uniquely presenting in O. chiliophylla. Although there were some slight differences in the chemicals of O. falcata and O. chiliophylla, the main components of both herbs were consistent generally. The results provided scientific basis, at least from the chemical point of view, for the reasonablity of two herbs being used as the same drug in Tibetan medicines and for the necessary of further investigation on their detailed chemical and pharmacological differences.本文首次建立了藏药"俄大夏"的两种基源药材—镰形棘豆和轮叶棘豆的一种简单、灵敏、可靠的高效液相色谱指纹图谱分析方法,并对不同产地以及不同时间采集的药材进行了分析,结果显示镰形棘豆有19个共有指纹峰,轮叶棘豆有24个共有指纹峰,镰形棘豆和轮叶棘豆有11个共有指纹峰。通过对照品对照指认了两种药材中共有的7个指纹峰以及轮叶棘豆特有的1个指纹峰的化合物结构。研究结果表明两种药材尽管在化学成分上存在一定差异,但是其主要成分基本相同,因此从化学成分的角度上为两种药材均作为"俄大夏"入药提供了科学依据,同时也提示为了保证临床用药的疗效和一致性,有必要对这两种药材的化学和生物活性区别进行深入细致的研究。

关 键 词：Oxytropis falcata Oxytropis chiliophylla High-performance liquid chromatographic fingerprint Quality analysis Tibetan medicines Er-Da-Xia 

分 类 号：R284.1[医药卫生—中药学]