半枝莲对铜绿假单胞菌群体感应系统的抑制作用  

Inhibition of the quorum sensing system in Pseudomonas aeruginosa PAO1 by Sculellaria barbata

作　　者：肖骏[1] 沈立新[1] 赵琳[1] 武玉婷[1] 段康民[1] 郭俏[1] 

机构地区：[1]西北大学生命科学学院,西部资源与现代生物技术省部共建教育部重点实验室,西安710069

出　　处：《中国抗生素杂志》2014年第12期885-890,共6页Chinese Journal of Antibiotics

基　　金：大学生创新创业训练计划项目(No.2012097);西部资源生物与现代生物技术教育部重点实验室开放基金资助(No.ES11007);国家自然科学基金面上项目(No.81171620);博士后科研启动基金(No.BSH13036)

摘　　要：目的在中药中筛选并研究铜绿假单胞菌(Pseudomonas aeruginosa,PA)群体感应系统抑制物(quorum-sensing inhibitor,QSI)。方法利用铜绿假单胞菌QS系统相关基因lasI、lasR、rhlI和rhlR的启动子与报道子荧光素酶(Luciferase)基因lux CDABE的融合子为筛选系统,通过检测化学发光反应不同中药对群体感应系统基因表达的影响,并检测中药对铜绿假单胞菌毒性相关表型的影响。结果研究结果表明中药半枝莲的水提物在不影响铜绿假单胞菌生长的条件下,可抑制PA中QS系统基因lasI、lasR、rhlI和rhlR的表达,并可抑制铜绿假单胞菌的运动性。结论中药半枝莲对铜绿假单胞菌群体感应系统有抑制作用,结果有助于新型抗生素的研究。Objective To screen for P.. aeruginosa quorum sensing inhibitors （QSI） from a range of Chinese herbs that are known for treating infections （i.e. Qing Re Jie Du）. Methods The promoters of QS （quorum sensing） genes （lasI, lasR, rhlI and rhlR） fused with luxCDABE reporter were used as the screen system. Both dick diffusion assay and liquid expression profiling were used. The expression of QS related gene clusters in the presence and absence of herbal extracts were monitored. Result The extract of Sculellaria barbara could inhibit QS genes, rhlI, rhlR, lasI and lasR in P. aeruginosa PAO 1, while had no influence on cell viability. Sculellaria barbara extract also repressed swarming and twitching motilities of P. aeruginosa PAO 1. Conclusion Sculellaria barbara extract shows inhibition on quorum-sensing in P. aeruginosa PAO 1. These results suggest new classes of antibiotics may be developed using Chinese herbs.

关 键 词：铜绿假单胞菌 群体感应系统 群体感应系统抑制剂 半枝莲 

分 类 号：R378[医药卫生—病原生物学]