空间诱变玉米自交系齐319的SSR标记变异分析  

SSR Analysis of Mutants from Maize ‘Qi319'Inbred Lines Induced by Space Mutagenesis

作　　者：于立伟[1] 张林[1] 李静[1] 邸宏[1] 刘显君[1] 曾兴[1] 张德贵[2] 李新海[2] 王振华[1] 

机构地区：[1]东北农业大学农学院,黑龙江哈尔滨150030 [2]中国农业科学院作物科学研究所,北京100081

出　　处：《核农学报》2014年第8期1345-1352,共8页Journal of Nuclear Agricultural Sciences

基　　金：黑龙江省教育厅科学技术研究项目(12531028)

摘　　要：玉米自交系齐319经"实践八号"育种卫星搭载后,发现两个籽粒诱变系SP-M1和SP-M2,由硬粒变为中齿和马齿。为解析籽粒变化原因,本文选用分布在玉米10条染色体上的385对SSR标记对两个变异株系和齐319进行变异分析。研究表明,SP-M1和SP-M2诱变系与齐319遗传相似系数分别为0.715和0.682,说明诱变系与野生材料之间存在遗传差异;中齿诱变系(SP-M1)与齐319之间检测到40个SSR变异位点,变异频率为10.93%,其中32个位点表现为扩增片段长度变异,8个位点表现为扩增片段数目变化;马齿诱变系(SP-M2)与齐319之间检测到55个SSR变异位点,变异频率为15.03%,其中47个位点表现为扩增片段长度变异,8个位点表现为扩增片段数目变化。不同染色体上位点变异频率差异较大,变异位点表现出成簇分布的特点,主要分布在第1、2、3、6、7、9、10条染色体上。本研究为探讨玉米空间诱变机制提供参考。SP-M1 and SP-M2 grain shape mutants were obtained from maize inbred line ‘Qi319＇ carried by satellite Shijian No. 8. The genetic differences between mutants and ‘Qi319＇ inbred line were studied based on SSR markers using 385 pairs of primers. The results showed that genetic similarity coefficients were 0. 715 between SP-M1 and ‘ Qi319＇ and 0. 682 between SP-M2 and‘Qi319＇ , respectively. There were 40 polymorphic loci between SP-M1 half- dent type mutant and‘Qi319＇, and the frequency of mutation was 10.93%. Among them, 32 loci showed polymorphism in the amplified fragment length, and 8 loci showed polymorphism in the number of the amplified fragment. We also found 55 polymorphic loci between SP-M2 dent type mutant and‘Qi319＇ , among which 47 loci showed polymorphism in amplified fragment length and 8 loci showed polymorphism in the number of the amplified fragment, and the frequency of mutation was 15.02%. The polymorphic loci were located on 1, 2, 3, 6, 7, 9 and 10 chromosomes, and distributed in clusters. This study provides a reference to explore space mutagenesis mechanism.

关 键 词：玉米自交系齐319 空间诱变 SSR分析 

分 类 号：S513[农业科学—作物学]