黄瓜AP1-FUL类MADS-box基因的克隆及表达分析  

Cloning and Expression Analysis of AP1- FUL Type MADS-box Gene in Cucumber( Cucumis Sativus L. )

作　　者：甘德芳[1] 吴娇[1] 庄丹[1] 梁丹笛 占梦丹 郝福玲[1] 

机构地区：[1]安徽农业大学园艺学院,安徽合肥230036

出　　处：《核农学报》2014年第9期1585-1590,共6页Journal of Nuclear Agricultural Sciences

基　　金：安徽省自然基金(1208085MC36);安徽省教育厅高等教育振兴计划项目(2013zdjy057);安徽农业大学引进和稳定人才项目(wd2011-14);安徽省高校省级优秀青年人才基金(2011SQRL050)

摘　　要：MADS-box基因在植物生长发育过程中起着非常重要的作用。利用生物信息学方法,获得黄瓜AP1-FUL类CsMADSs基因;半定量RT-PCR检测CsMADSs在黄瓜不同生长发育阶段及不同器官中的表达情况。结果表明,本研究克隆得到三个CsMADSs基因,长度分别为636bp、747和648bp。CsMADS08和CsMADS10在黄瓜不同时期的不同器官中均能表达,其中,CsMADS08在茎和叶中的表达量较高,而CsMADS10在顶芽中表达量较高;CsMADS21基因除了在1叶和5叶期的各器官中都能表达外,在其他器官只是部分表达,且表达量都比较低。另外,CsMADS08和CsMADS10几乎在7叶期黄瓜的所有器官中都表达,而CsMADS21只在少数器官中表达,以幼果中表达量较高,而在根、茎及较大的卷须中都不表达。该研究结果不仅能够明确黄瓜CsMADSs基因的时空表达模式,而且为进一步研究CsMADSs基因的功能提供理论依据。MADS-box genes have important functions in the regulation of plant growth and development. By bioinformatics methods and sequence analysis, CsMADS genes of AP1 - FUL type were observed. Expression analysis of CsMADSs gene was observed in different growth stages and different organs in cucumber. Results showed that three CsMADS genes were cloned and the eDNA lengths were 636bp, 747bp and 648bp respectively. CsMADS08 and CsMADSIO expressed almost in all organs and all periods, among which, high expression of CsMADS08 was detected in the stems and leaves while high expression of CsMADSIO was in the shoot apex. CsMADS21 expressed only in a few organs, and the expression were low except for young fruits. In addition, the expression of three CsMADSs gene was also different in different organs of the 7 - leaf-stage cucumber. CsMADS08 and CsMADSIO could express in all organs, while CsMADS21 expressed only in a few organs and especially high expression in young fruits. These results can not only allow us understand the expression pattern of CsMADSs gene in cucumber, but also provide some significant theoretical basis for further research on the function of CsMADSs gene.

关 键 词：黄瓜 MADS-BOX 卷须 克隆 转录因子 表达分析 

分 类 号：S642.2[农业科学—蔬菜学]