植物DNA错配修复缺陷及其对诱变育种的意义  

Plant Mismatch Repair Deficiency and Its Significance for Mutation Breeding

作　　者：崔海瑞[1] 宋悦[1] 袁兵[1] 赵海军[1] 富昊伟[2] 舒庆尧[1] 

机构地区：[1]浙江大学原子核农业科学研究所/农业部核农学重点开放实验室,浙江杭州310029 [2]嘉兴市农业科学研究院,浙江嘉兴314016

出　　处：《核农学报》2014年第9期1606-1614,共9页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金项目(1175154);农业公益性行业科研专项(201103007);IAEA协调研究项目(CPR15655)

摘　　要：DNA错配修复(MMR)是DNA损伤修复的一个重要途径,主要司职于DNA合成、遗传重组及损伤过程中新发生的单个及少数碱基的缺失、插入及错配的修复,对维持基因组稳定性和DNA复制保真度至关重要。原核生物的MMR系统主要由MutS、MutH和MutL组成,在高等植物中也鉴定出MutS和MutL的同源物,但未发现MutH。本文介绍了近年来有关植物DNA错配修复及相关基因功能的研究进展,探讨了植物MMR功能缺陷产生的途径及其对植物诱变育种的意义,为进一步利用MMR缺陷突变体进行植物诱发突变与诱变育种研究提供借鉴。The mismatch repair （MMR） is a major pathway in DNA repair system, which is critical for maintaining genome stability and DNA fidelity in the replication, as it is responsible for recognizing and repairing erroneous insertions, deletions and mismatch bases newly arisen during DNA replication and genetic recombination, as well as during repair of some forms of DNA damage. The major components in the MMR system include MutS, MutH and MutL in Escherichia coli. The homologues of both MutS and MutL have been found in higher plants, but none of MutH. This review focuses on the research progress in MMR system and the function of MMR genes in plants, highlights the production way of MMR functional deficiency and its significance for plant induced mutation breeding. It is attempted to provide a reference for researches on further application of MMR deficient mutants in plant mutagenesis and mutation breeding.

关 键 词：植物 错配修复 功能缺陷 诱变育种 

分 类 号：Q943.2[生物学—植物学]