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作 者:胡滨[1] 陈一资[1] 胡惠民[1] 李诚[1] 杨勇[1] 何利[1] 苏赵[1]
出 处:《核农学报》2014年第9期1632-1640,共9页Journal of Nuclear Agricultural Sciences
基 金:四川省"十二五"重大科技攻关课题(2012NZ001)
摘 要:本文主要研究猪血蛋白酶解物的抗氧化作用。通过其对超氧阴离子自由基、羟自由基、1,1-二苯基-2-三硝基苯肼自由基的清除能力以及还原能力的测定,进行体外抗氧化试验;以溴代苯致小鼠试验性肝脏过氧化损伤为模型,进行体内抗氧化试验。结果表明,在体外抗氧化试验中,猪血蛋白酶解物对化学体系产生的自由基、羟自由基、DPPH自由基有较强清除能力,同时还具有还原能力;在体内抗氧化试验中,猪血蛋白酶解物对溴代苯所致的试验性肝脏过氧化性损伤起保护作用。由此可知猪血蛋白酶解物具有抗氧化作用。本文为广泛利用猪血蛋白资源和开发新的抗氧化补充剂提供依据。The object of this study is to investigate the antioxidant activity of porcine blood hydrolysate (PBH). The antioxidant activity of PBH in vitro was evaluated by determining the abilities to scavenge DPPH, hydroxyl and superoxide anion free radicals as well as reducing power. A mouse model of liver oxidative damage induced by Bromobenzene in vivo were used. The result shows that PBH had relatively strong scavenging power for DPPH, hydroxyl and superoxide anion free radicals, and possessed reducing power in vitro experiment; that PBH had protective function for the liver' s damage caused by Bromobenzene in vivo experiments. So, PBH had antioxidant activities. This research can offer the basis to wide usage of porcine blood and enploration of antioxidants.
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