14个赖草属植物DREB2基因的克隆与SNP分析  

Cloning and SNP Analysis of DREB2 Gene in Fourteen Leymus Species

作　　者：高刚[1] 邓家彬[1] 苟学梅 王茜[1] 张艳[1] 杨瑞武[1] 

机构地区：[1]四川农业大学生命科学学院,雅安625014

出　　处：《植物研究》2015年第1期47-51,共5页Bulletin of Botanical Research

基　　金：国家自然科学基金(No.30870154)

摘　　要：植物的DREB2转录因子具有抗旱、抗盐碱的功能。对小麦族赖草属的14个物种的DREB2基因进行了克隆、测序和比对分析。结果表明:(1)14个赖草属植物的DREB2基因序列和氨基酸序列相对保守,总相似度分别为98.05%和97.78%;(2)Leymus mollis的DREB2基因在编码区有3 bp CTT的缺失;(3)14条DREB2基因序列共发现79个SNP,SNP发生频率为1SNP/10.6 bp;(4)进化树分析显示DREB2基因能把14个赖草属物种很好的分开。DREB2 transcription factor plays an important role in response to abiotic stresses ot drought and salinity. We obtained the DREB2 sequences from 14 Leymus species. By sequence analysis, the DREB2 gene and amino acid are conserved among 14 Leymus species. The similarity of nucleotide sequence and amino acid sequence are 98.05% and 97.78% , respecctively. There are 3 bp deletion CTr in L. mollis. Among these sequences, there are 79 SNP identified. The polymorphism frequence is 1SNP/10.6 bp. Fourteen Leyrnus species can be well separated by the phylogenetic trees of DREB2 gene.

关 键 词：赖草属 DREB2 单核苷酸多态性(SNP) 序列分析 

分 类 号：Q943.2[生物学—植物学]