抗寒杂交榛子微体繁殖技术研究  

A Micro-propagation Technique of Corylus heterophylla × C. avellana

作　　者：张玲 

机构地区：[1]黑龙江省林业科学研究所,哈尔滨150081

出　　处：《植物研究》2015年第1期146-149,共4页Bulletin of Botanical Research

基　　金：黑龙江省森工总局项目(SGJZQ2012002)

摘　　要：以抗寒评比优良杂交榛子的叶片、茎段、腋芽、子叶为材料,采用不同基本培养基和不同激素及浓度组合,探讨各种处理对抗寒杂交榛子微体繁殖体系的影响。结果表明:(1)采用经抗寒性评比优良的杂交F1代10个优良品种进行组织培养,筛选出3个组织培养效果优良的品种84-226、84-310、84-253;(2)采用DKW、MS、WPM三种培养,筛选出WPM最适合抗寒杂交榛子愈伤组织诱导的培养基、MS为最适合增殖培养的培养基;(3)愈伤组织诱导基本培养基配方为WPM+6-BA 2.0 mg·L-1+NAA 1.5 mg·L-1培养,腋芽扩繁以MS改良培养基苗生长状况较好,激素为6-BA 1.5 mg·L-1+NAA 0.1 mg·L-1。该研究可以解决寒地杂交榛子繁殖系数低,为进一步试管苗的工厂化生产提供理论依据。In cold resistance appraisal of fine varieties hybrid hazelnut leaves, stems, axillary bud cotyledons, we studied various treatments on cold resistance hybrid hazelnut micro-propagation system with different culture medium, hormones and contents. By using ten tissue cultures of a fine varieties hardiness of F1 hybrid varieties, we selected the effect of three superior varieties 84-226, 84-310, 84-253. By using three culture medium of DKW, MS and WPM, WPM was more suitable for cold resistance hazelnut callus induction medium, and MS was the most suitable medium culture propagation. The basic training callus induction based formulations was cultured in WPM ＋ 6-BA 2.0 mg· L - 1 ＋ NAA 1.5 mg · L - 1, and axillary bud propagation was in MS in good condition with the hormone of 6-BA 1.5 mg · L- 1 ＋ NAA 0.1 mg · L-1. Our study can be used in cold resistance of hybrid hazelnut low coefficient, and can provide a theoretical basis for further industrial production plantlets.

关 键 词：抗寒杂交榛子 微体繁殖 

分 类 号：S664.4[农业科学—果树学]