石蜡切片法中染色技术的改良  

The Improved Stained Methods of Paraffin Sections Production

作　　者：王秀文[1] 

机构地区：[1]齐齐哈尔大学生命科学与农林学院,齐齐哈尔161000

出　　处：《植物研究》2015年第1期158-160,共3页Bulletin of Botanical Research

摘　　要：石蜡切片是观察动植物组织最直接有效的方法之一。本实验对水稻品种9311的叶片进行切片并用1%的番红—乙醇染液和1%的苯胺蓝—乙醇染液进行染色,结果显示此改良方法得到的组织比传统石蜡切片更完整,染色的效果比传统石蜡切片更清晰,并且利用此方法对水稻叶片进行制片,可以大量缩短制片后期复水及脱水的时间,提高工作效率。Paraffin section is one of the most direct and effective way to observe plant and animal tissues. We sliced rice leaf of 9 311 and stained with 1% Safranin-ethanol solution and Aniline blue-ethanol solution. The organization of our improvement is more complete than traditional paraffin , stained effect is more clearly than traditional method, and sliced rice leaf in this method can significantly shorten the rehydration and dehydration time in the late production and improved work efficiency.

关 键 词：石蜡切片 水稻叶片 染色技术 改良 

分 类 号：Q94[生物学—植物学]