MSPD-HPLC法测定金线吊乌龟中千金藤素  

Determination of cepharanthine in Stephania cepharantha Hayata by MSPD-HPLC

作　　者：汪亚勤[1] 殷嘉珺[1] 谢道涛 康云[1] 翁伟宇[2] 黄建明[1] 

机构地区：[1]复旦大学药学院,上海201203 [2]华东理工大学,上海200237

出　　处：《中成药》2014年第12期2557-2560,共4页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金项目(31100238);复旦大学青年教师科研能力提升项目(20520133180)

摘　　要：目的建立基质固相分散-高效液相色谱法（MSPD-HPLC）测定千金藤属植物金线吊乌龟块根中千金藤素的量。方法样品的前处理采用MSPD法,以碱性氧化铝为吸附剂,甲醇为洗脱溶剂。HPLC分析采用Phenomenex Gemini C18色谱柱（250 mm×4.60 mm,5μm）,流动相为乙腈-缓冲液（0.5%三乙胺水溶液用磷酸调节p H至10.7）,体积流量1.0 m L/min,检测波长282 nm,柱温40℃。结果千金藤素在14.88-238μg/m L范围内呈良好线性关系（r=0.999 4）,平均回收率为98.2%,RSD 3.0%（n=6）。结论所建MSPD-HPLC法简便、快速,可用于金线吊乌龟中千金藤素的定量分析。AIM To establish a matrix solid-phase dispersion（ MSPD） extraction method followed by HPLC for the determination of cepharanthine in Stephania cepharantha Hayata. METHODS The samples were prepared by an optimized MSPD procedure using basic alumina as the sorbent and methanol as the elution solvent. The HPLC separation was carried out on a Phenomenex Gemini C18column（ 250 mm × 4. 60 mm,5 μm） with an isocratic mobile phase consisting of acetonitrile-0. 5% triethylamine aqueous solution adjusted to p H 10. 7 with phosphoric acid（ 70 ∶ 30）. The flow rate was 1. 0 m L / min,the detection wavelength was set at 282 nm,and the column temperature was 40 ℃. RESULTS The calibration curve of cepharanthine was linear in the range of14. 88 ~ 238 μg / m L（ r = 0. 9994）. The average recovery was 98. 2% with RSD 3. 0%（ n = 6）. CONCLUSION The MSPD-HPLC method is simple,rapid,and applicable to the quantification of cepharanthine in S. cepharantha.

关 键 词：金线吊乌龟 千金藤素 基质固相分散法 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]