不同环境下军人肠道大肠埃希菌耐药性分析  

Drug resistance of intestinal Escherichia coli isolates from soldiers under different environments

作　　者：张樱[1] 周光[1] 杨继勇[1] 张有江[1] 王蕊[1] 叶丽艳[1] 郭玲[1] 罗燕萍[1] 

机构地区：[1]解放军总医院微生物,北京100853

出　　处：《中华医院感染学杂志》2014年第24期6009-6011,共3页Chinese Journal of Nosocomiology

基　　金：军队后勤科研“十二五”重大项目(AWS11C001)

摘　　要：目的了解不同环境下军人正常肠道大肠埃希菌(ECO)分布及其耐药性,评估健康青壮年肠道耐药ECO的携带情况。方法采集2013年11月南方某连队152名军人正常粪便,对分离的128株ECO采用VITEK MS进行菌株鉴定,参照CLSI 2013版推荐的微量肉汤稀释法进行药敏试验,应用WHONET5.6和EXCEL软件分析细菌分布和耐药性。结果 152人中共分离出128株ECO占84.2%,其中59名海上工作人员分离到47株ECO占79.7%,产超广谱β-内酰胺酶ECO(ESBLs-ECO)5株占10.6%;63名陆地警卫人员分离到57株ECO占90.5%,ESBLs-ECO 5株占8.8%;30名炊事员分离到24株ECO占80.0%,ESBLs-ECO 8株占33.3%;海上工作人员、陆地警卫人员和陆地炊事员3组之间ESBLs-ECO阳性率差异有统计学意义;所有菌株对哌拉西林/他唑巴坦、头孢吡肟、亚胺培南和美罗培南均敏感。结论长期暴露在食品加工环境中的工作人员,有更多机会接触到通过食物链传播的耐药菌,因此,畜牧业的抗菌药物使用应得到更加严格控制。OBJECTIVE To understand the distribution and drug resistance of intestinal Escherichia coli isolates from the soldiers under different environments and evaluate the prevalence of the drug‐resistant E .coli isolates in the intestinal tracts of young adults .METHODS The normal stool specimens were collected from 152 soldiers from a military company in southern China in Nov ,2013 ,then 128 strains of isolated E .coli were identified by using VITEK MS ,the drug susceptibility testing was performed with the use of the micro‐broth dilution method recom‐mended by CLSI 2013 edition ,and the distribution and drug resistance of the strains were analyzed by using WHONET5 .6 software and EXCEL software .RESULTS Totally 128 E .coli isolates have been isolated from the 152 participants ,accounting for 84 .2% ,among which 47 （79 .7% ） strains were isolated from 59 navy ,and there were 5 （10 .6% ） strains of extended‐spectrum β‐lactamases （ESBLs）‐producing E .coli .Totally 57 E .coli isolates have been isolated from 63 land guards ,accounting for 90 .5% ,among which 5 strains of ESBLs‐producing E .coli accounted for 8 .8% .Totally 24 E .coli isolates have been isolated from 30 cooks ,accounting for 80 .0% ,among which 8 strains of ESBLs‐producing E .coli accounted for 33 .3% ;there was significant difference in the positive rate of ESBLs‐producing E .coli between the navy ,land guards ,and cooks .All the isolated strains were suscepti‐ble to piperacillin‐tazobactam ,cefepime ,imipenem ,and meropenem .CONCLUSION The staff who have long‐term exposure to food processing environment are prone to carry the drug‐resistant strains that are transmit ted through the food chain ,therefore ,it is necessary to use antibiotics more reasonably for animal husbandry .

关 键 词：军人 大肠埃希菌 分布 耐药性 

分 类 号：R378.21[医药卫生—病原生物学]