水稻OsGL1-2基因反义表达载体的构建  

Construction of Plant Antisense Expression Vector for Os GL1-2 Gene in Rice

作　　者：黄丹莹[1,2] 叶能辉[2] 庄楚雄[2] 

机构地区：[1]揭阳职业技术学院,广东揭阳522000 [2]华南农业大学生命科学学院,广东广州510642

出　　处：《安徽农业科学》2014年第36期12818-12820,共3页Journal of Anhui Agricultural Sciences

基　　金：广东省揭阳市揭阳职业技术学院科研基金项目(JYCKY0904)

摘　　要：应用聚合酶链式反应技术(PCR)扩增水稻Os GL1-2基因反义片段及基因自身的启动子,并分别克隆到p UC19克隆载体上,得到含有Os GL1-2启动子+Os GL1-2反义片段的中间载体。对重组子进行PCR检测和酶切分析并测序,结果表明,长度分别为417和2 199bp。将Os GL1-2启动子+Os GL1-2反义片段克隆到植物表达载体p Cambia1380多克隆位点,构建了该基因的植物反义表达载体p Cam GL1-2。OsGL1-2 antisense fragment and its promoter were amplified by PCR technique and cloned into the same pUC19 clone vector. ＇lherecombinant clones were detected by both PCR technique and restriction enzymes. The OsGL1-2 antisense fragment and promoter were se-quenced. Results showed that the lengths of the both DNA fragments were 417 bp and 2199 bp, respectively. The OsGL1-2 promoter ＋ OsGL1-2 antisense fragment fi＇om intermediate vector pUC19 was cut off and cloned into the multiple cloning sites of the plant expression vector pCam-bia1380, which can be used as antisense expression vector pCamGLl-2 for down-regulation of OsGL1-2 gene.

关 键 词：水稻 GL1-2 启动子 反义表达载体 GL1-2 

分 类 号：Q782[生物学—分子生物学]