鸭大肠杆菌自家铝胶灭活苗的制备及质量检验  

Preparation and Quality Examination of Self-prepared Aluminum Hydroxide Inactivated Vaccine against Escherichia coli of Duck

作　　者：刘玉华[1] 方向红[1] 王琳琳[1] 戴丽红[1] 

机构地区：[1]江苏农牧科技职业学院,江苏泰州225300

出　　处：《安徽农业科学》2014年第36期12929-12930,共2页Journal of Anhui Agricultural Sciences

摘　　要：[目的]为有效控制鸭大肠杆菌病的流行提供参考依据。[方法]利用分离自泰州某规模化养鸭场的大肠杆菌菌株TZY1作为菌种,制备鸭大肠杆菌铝胶灭活苗,并进行相应的质量检验。[结果]通过无菌检验、安全检验和动物保护力试验发现自制疫苗无外源性微生物污染,对雏鸭安全性好。对雏鸭的攻毒试验结果表明制备疫苗的免疫保护率达100%,该疫苗的免疫剂量为0.5 ml。田间试验结果表明免疫保护率达95.1%。[结论]成功制备了鸭大肠杆菌铝胶灭活苗。[ Objective ] The research aimed to provide reference basis for controlling the prevalence of duck colibacillosis effectively. [ Meth-od ] TZY1 strain of Escherichia coli separated from a large-scale duck farm in Taizhou was used to prepare the aluminum hydroxide inactivatedvaccine against Escherichia coli of duck. And its quality indices were examined. [Result] The results of sterilization test, safety examinationand animal protection test showed that the self-prepared vaccine wasn＇ t polluted by exogenous microorganisms and it had good safety to duck-lings. The results of challenge experiment on ducklings showed that the immune protection rate of the self-prepared vaccine reached 100% andits immunization dose was 0.5 ml. The results of field experiment shoed that the immune protection rate was 95.1%. [ Conclusion] Aluminumhydroxide inactivated vaccine against Escherichia coli of duck was successfully prepared.

关 键 词：鸭 大肠杆菌 铝胶 灭活苗 制备 

分 类 号：S834[农业科学—畜牧学]