2009～2013年临床分离病原菌分布及耐药性分析  

Distribution and resistance of clinical isolates from clinical specimans from 2009 to 2013

作　　者：王俊妨[1] 靳颖[1] 刘锦[1] 周鑫[1] 

机构地区：[1]武警后勤学院附属医院检验科,天津300612

出　　处：《检验医学与临床》2014年第A02期17-20,共4页Laboratory Medicine and Clinic

摘　　要：目的了解该院2009～2013年临床病原菌的分布情况及耐药性分析，为临床医生合理选择抗菌药物提供依据。方法对2009～2013年临床科室送检的培养标本，采用MicroScan Walkway96及VITEK Compact1I全自动细菌鉴定及药敏分析仪进行鉴定及药敏试验，并对其结果采用WHONET5.4进行统计分析。结果共分离出病原菌45345株，培养阳性率平均为41.42％。其中革兰氏阳性菌占23.89％（10833株），革兰氏阴性菌占67.51％（30613株），真菌占8.6％（3899株）。分离菌株中最常见的菌种依次为肺炎克雷伯菌、铜绿假单胞菌、金黄色葡萄球菌、鲍曼不动杆菌、大肠埃希菌等。耐甲氧西林的金黄色葡萄球菌（MRSA）占74.04％，产ESBLs酶的肺炎克雷伯菌及大肠埃希菌分别占46.35％及40.37％，铜绿假单胞菌和鲍曼不动杆菌多重耐药或泛耐药菌株分离率为20.1％、24.5％。白色假丝酵母菌分离率为74.1％。结论该院临床分离菌株呈上升趋势，分离的菌株耐药率比较高，多重耐药或泛耐药菌株逐年递增，应加强细菌耐药监测、控制院内感染，降低医疗成本，减少医疗资源耗费、缩短患者住院时间。Objective To investigate the bacterial resistance of clinical isolates from the Affiliated Hospital of Medical Colleage of The Chinese People＇s Armed Police Forces from 2009 to 2013, to apply the basis for clinical. Methods All strains collected were identified and the antimicrobial susceptibility were determined with MicroScan Walkway 96 and VITEK Compact Ⅱ ,The data were analyzed by WHONETS. 4 software. Results 45345 clinical i- solates separated from clinical samples. Gram positive organisms accounted for 23.89%, and gram negative organisms accounted for 67.51%. The most common strain were Klebsiella Pneumoniae,Pseudomonas Aeruginosa,Staphylococ- cus Aureus,Acinetobacter Baumannii,Escherichia coil etal. The rate of Methicillin Resistant Staphylococcus Aureus （MRSA）reached to74.04%, The rates of Extended Spectrum β-lactamases（ESBLs）in Klebsiella Pneumoniae and Escherichia coil were 46.35% and 40.37%. The multi-drug resistant isolates of Pseudomonas Aeruginosa and Acine- tobacter Baumannii were 20.1% and 24.5%. The isolation rate of C. albicans was 74.1%. Conclusion The clinical i- solates from our hospital were high separation rates and high drug resistance levels. We should monitor the drug-re- sistance levels,control Nosocomial infection, reduce the cost of medical and the waste of medical resources, shorten the time of hospitalization.

关 键 词：病原菌 抗菌药物 耐药性 

分 类 号：R378[医药卫生—病原生物学]