应用响应面分析优化酶法辅助超声提取余甘子多糖工艺  

Optimization of enzyme-ultrasonic extraction of polysaccharides from Phyllanthus emblica L. using response surface analysis

作　　者：罗兰[1] 曾茂贵[2] 饶雪娥 李羚羚[1] 

机构地区：[1]福建卫生职业技术学院药学系,福建福州350101 [2]福建中医药大学附属第二人民医院,福建福州350003

出　　处：《亚热带农业研究》2014年第4期264-268,共5页Subtropical Agriculture Research

基　　金：福建卫生职业技术学院院级课题(2012-2-6)

摘　　要：利用酶法辅助超声提取法从余甘子中提取多糖,考查了超声时间、液料比、超声温度和超声功率对多糖提取率的影响。在单因素试验的基础上采用响应面分析优化提取工艺条件。结果表明,余甘子多糖的最佳提取工艺条件为:超声温度41℃,液料比6∶1,超声时间75 min,超声功率300 W,多糖提取率为5.76%,接近于模型预测值。The optimization of the extraction process of polysaccharides from Phyllanthus emblica L. by ultrasonic combined with enzymic method has been determined by response surface analysis( RSA) after investigating the effects of time,ultrasonic power,temperature and liquid solid ratio on the extraction yield of polysaccharide based on the single-factor experiments. The optimum extraction yield was 5. 76% when the temperature,time and proportion were 41 ℃,75 min,and 6∶ 1( m L∶ g) respectively. This study showed the process was feasible and reproducible.

关 键 词：余甘子 多糖 酶法辅助超声提取 单因素试验 响应面分析 

分 类 号：R285[医药卫生—中药学] R965[医药卫生—中医学]