金银花-山银花水提液不同配比体外抗氧化作用研究  

Study on antioxidant activity of water extracts from different proportions of honeysuckle flower and wild honeysuckle flower in vitro

作　　者：田磊[1] 蒋宝平[2] 何苗[2] 李申[3] 樊晓峰[2] 

机构地区：[1]南京中医药大学附属医院,南京210029 [2]南京中医药大学,南京210023 [3]河南护理职业学院,安阳455000

出　　处：《实用药物与临床》2014年第10期1290-1294,共5页Practical Pharmacy and Clinical Remedies

基　　金：国家自然科学基金青年基金(81102885);江苏省中医药局科技项目(LZ11194)

摘　　要：目的观察金银花、山银花水提液不同配比(1∶0、2∶1、1∶1、1∶2、0∶1)对3种自由基的清除作用,以及对H2O2诱导的内皮细胞氧化损伤的保护作用。方法采用1,1-二苯基-2-苦肼基自由基(DPPH·)、羟基自由基(·OH)、超氧阴离子自由基(O2-·[O])清除法测定金银花—山银花水提液5个不同配比组的抗氧化活性;采用体外培养HUVECs细胞并复制细胞损伤模型,测定金银花—山银花水提液5个不同配比组受损细胞的活力、受损细胞上清中乳酸脱氢酶(LDH)和一氧化氮(NO)的含量。结果金银花—山银花水提液不同配比均有一定的抗氧化作用,其中金银花—山银花2∶1组最佳。金银花—山银花水提液不同配比能够使受损细胞NO的含量增加、LDH释放减少,其中金银花—山银花1∶0组作用最强,2∶1组次之。结论金银花—山银花不同配比在体外有较强的抗自由基和抗氧化作用,并且能够保护损伤的HUVECs细胞,金银花-山银花2∶1综合效果最佳。Objective To observe the scavenging effect on 3 kinds of different freedom radicals and the protective effect against the damage caused by hydrogen peroxide（H2O2） in endothelial cells of water extracts from honeysuckle flower and wild honeysuckle flower in different proportions（1∶ 0,2∶ 1,1∶ 1,1∶ 2,0∶ 1）. Methods The scavenging ability of 5 different proportions of water extracts from honeysuckle flower and wild honeysuckle flower on 1,1-diphenyl-2-trinitro bitter hydrazine radical（DPPH·）,hydroxyl radical（·OH）,superoxide anion radical（ O2-·） was determined to evaluate the antioxidant activity of the water extracts from honeysuckle flower and wild honeysuckle flower in different proportions. HUVECs were cultured in vitro to build the hypoxic-reoxygenation model,then the cell viability was determined by MTT,and the lactate dehydrogenase（LDH） and nitrogen monoxide（NO） level in cell culture medium of 5 kinds of different proportions of water extracts from honeysuckle flower and wild honeysuckle flower were detected. Results All of the different proportions had the antioxidant effect,and the honeysuckle flower and wild honeysuckle flower 2∶ 1 group had the best effect. All different proportions could effectively release the contention of NO and reduce LDH release from damaged cells,and the honeysuckle flower and wild honeysuckle flower 1∶ 0 group had the best effect,followed by honeysuckle flower and wild honeysuckle flower 2∶ 1 group. Conclusion All different proportions of water extracts from honeysuckle flower and wild honeysuckle flower have obviously anti free radical and antioxidant effects in vitro,and have protective effect on human umbilical vein endothelial cells injury,and the honeysuckle flower and wild honeysuckle flower 2∶ 1 group had the best effect.

关 键 词：金银花 山银花 抗氧化 

分 类 号：R284[医药卫生—中药学]