三例眼部突变小鼠的培育及特征分析  

Breeding and Phenotyping Three Cases of Eye Disease Mice

作　　者：杨伟伟[1] 金红颖[2] 吴晓玲[1] 周祥[1] 李高天[1] 刘桂杰[1] 顾美儿[1] 俞利平[1] 宋晓明[1] 吴宝金[1] 

机构地区：[1]杭州师范大学实验动物科学实验室,杭州310036 [2]浙江大学第二附属医院眼科,杭州310009

出　　处：《实验动物科学》2014年第5期1-4,I0001,I0002,共6页Laboratory Animal Science

基　　金：浙江省公益性技术应用研究计划项目(No.2012C37084)

摘　　要：目的筛选培育眼部突变表型小鼠,为人类相关疾病的研究提供材料。方法采用N-乙基-N-亚硝基脲(N-ethyl-N-nitrosourea,ENU)诱变处理G0代小鼠,繁育获得G1代,筛选眼部突变表型个体,进行遗传力试验、临床诊断及病理学观察。结果本实验繁殖G1代小鼠2782只,经筛查获得眼部突变表型小鼠65只,可稳定遗传小鼠3例,分别表现为:角膜混浊、小眼球和虹膜异常等特征。角膜混浊者其角膜症状严重程度差异较大,角膜病变部位明显增厚,部分伴有新生血管;小眼球者睑裂较小,甚至上下眼睑粘连,外观眼球不可见,病理学检查可见内有发育异常的小眼球;虹膜异常者可见瞳孔偏大,明显偏离中心位置,偏向位置不定,对光无反射,病理学观察可见虹膜晶状体粘连、虹膜缺损,严重者伴有视网膜异常等。结论本实验成功培育了3例眼部突变表型小鼠,为人类相关疾病的研究提供良好的材料。In order to obtain materials of eye-mutant mice for human eye related diseases research, generation zero （G0） mice were mutagenized by N-ethyl-N-nitrosourea （ENU） , and G1 generation mice were bred and selected for eye mutant phenotype. All mice were proceeded heritabilitytest, clinical diagnosis and pathological observation respectively. A total of 2782 mice were bred and 65 eye mutants were screened, in which, three cases mice were inheritable, and congenital corneal opacity, nanophthalmos and iris malformation were its phenotypic characteristic respectively. Corneal opacity turbidity was fairly different, especially in terms of hyperplasia and new angiogenesis. The phenotypic traits of nanophthalmos mice was blepharophimosis, blepharosymphysis, and the eyeball was invisible （severe, general inspection）, but dysplastic nanophthalmos was visible （pathological observation）. The phenotypic characteristic of iris malformation was found that the pupil was larger, obvious deviation from the central location, and no light reflection, irido-lens adhesions, collyriculum iridis, retina abnormality ea tl. Three cases of ENU-induced eye mutant mice were bred successfully and lay a foundation for human eye disease research.

关 键 词：眼部疾病 小鼠模型 诱变 

分 类 号：Q95-33[生物学—动物学]