白色念珠菌与细菌混合感染小鼠阴道炎动物模型的构建及感染菌的鉴定  

Construction of Animal Vaginitis Models by Mixing Candida albicans with Bacteria and Identification of Infect Bacteria

作　　者：胡日新[1] 潘立博[1] 姜新[2] 王靖宇[1] 王福金[1] 

机构地区：[1]大连医科大学实验动物中心,大连116044 [2]通化师范学院分院,通化134000

出　　处：《实验动物科学》2014年第5期5-9,27,I0002,共7页Laboratory Animal Science

摘　　要：目的在制作单一白色念珠菌性阴道炎动物模型的过程中,出现了两例严重感染的小鼠,因此,对其感染机制进行了深入研究。方法从严重感染小鼠的阴道内分离得到真菌与细菌,经纯化3代后得到15个真菌样本和19个细菌样本,对于真菌样本进行念珠菌的显色培养基鉴定,对于细菌进行了16Sr DNA提取、PCR验证和PCRDGGE分析,对不同种细菌的DNA样本进行序列测定。结果分离出1种真菌和4种致病性细菌,证实了小鼠阴道重度感染是由1种真菌和4种细菌共同作用而引起。结论我们所发现的小鼠阴道重度感染模型,是由白色念珠菌(Monilia albican)与4种细菌,即变形菌(Proteus vulgaris)、嗜麦芽寡养单胞菌(Stenotrophomonas maltophilia)、产碱杆菌(Alcaligenes Castellani&Chalmers)和芽孢杆菌(Bacillus)的混合感染所致。Objective In the process of making animal models with single Monilia albicans vaginitis, there weretwo cases of serious infection in mice, so the infection mechanism were studied deeply. Method Isolating fungi and bacteria from mice vagina with severe infection, the 15 fungal samples and 19 samples of bacteria were got after purifying for 3 generations. For fungal samples, we conducted chromogenic medium identification of Monilia albicans. For samples of bacteria, we conducted 16SrDNA extraction, PCR validation and PCR-DGGE analysis, besides we conducted sequence determination of different bacteria DNA samples. Result We isolated 1 kind of fungi and 4 kinds of pathogenic bacteria, it confirmed that the mouse vaginal severe infection is caused by the interaction of 1 kind of fungi and 4 kinds of bacteria. Conclusion The mouse vaginal severe infection model we found, is caused by the mixed infection of 1 kind of fungi： Monilia albican and 4 kinds of bacteria： Proteus vulgaris, Stenotrophomonas maltophili, Alcaligenes Castellani＆Chalmers and Bacillus.

关 键 词：白色念珠菌 细菌 混合感染 阴道炎 动物模型 

分 类 号：Q95-33[生物学—动物学]