大黄牡丹汤组方对急性胰腺炎模型大鼠炎症反应及氧化应激水平的影响  

The Effect of Dahuang Mudan Decoction on Inflammatory Response and Oxidative Stress in Acute Pancreatitis Model Rats

作　　者：张延英[1] 舒畅 蔡兴[2,3] 吴建军[1] 张艳霞[1] 王茸[1] 李存祥[1] 康万荣[1] 王荣[1] 

机构地区：[1]甘肃中医学院/甘肃省中药药理与毒理学重点实验室/甘肃省高校中(藏)药化学与质量研究省级重点实验室,兰州730000 [2]甘肃省分析测试中心 [3]甘肃省实验动物学会,兰州730000

出　　处：《实验动物科学》2014年第5期24-27,共4页Laboratory Animal Science

基　　金：甘肃省技术研究与开发专项(No.1305TCYA043)

摘　　要：目的研究大黄牡丹汤组方(Rhei radix et rhizome peony Decoction Prescription,RPDP)对急性胰腺炎(AP)模型大鼠炎症反应及氧化应激水平的影响。方法 SD大鼠分为假手术组、模型组和治疗组。以0.1 m L/min的速度,经十二指肠壁浆肌层穿刺距离胆胰管开口0.3 mm处的胆管,加压注射3.5%牛磺胆酸钠0.1 m L/100 g,建立大鼠AP模型。治疗组造模后立即给予RPDP 40 g/kg灌胃1次,假手术组、模型组给予等量的生理盐水灌胃1次。各组分别于造模后4h、8h、12h处死10只大鼠并取血,检测血清中IL-6、IL-β和TNF-α含量;同时切取各时段大鼠胰腺组织,检测MDA、MPO和SOD的含量。结果与模型组相比,治疗组各时段血清IL-6、IL-β和TNF-α含量均显著减少(P<0.05);各时段胰腺组织中MDA、MPO含量显著降低(P<0.05),SOD含量显著升高(P<0.05)。结论 RPDP有抑制TNF-α、IL-6、IL-β的释放,减少MDA产生,有抗过氧化损伤作用,提高机体对OFR的清除能力。Objective To study the effect of Dahuang Mudan Decoction （RPDP） on inflammatory response and oxidative stress in acute pancreatitis（AP） model rats. Method Divided 90 SD rats randomly into three groups： the sham operation（SO） group , the AP group, and the RPDP group. The models of AP were made by pressurize inject 3% sodium taurocholate into the bile duct where was 0.3ram away from the pancreatic duct with the speed of O. lml/min. The rats in the RPDP group were lavaged with RPDP （40 g/kg） after made AP model. The rats in the SO group and the AP group were lavaged with partes aequales Sodium Chloride. Sacrifice the rats 4 h, 8 h, 12 h hours after establish the animal model, separated the serum and detected the content of IL-6, IL-β and TNF-α in the serum, cut pancreatic tissue in every time quantum and detected the content of MDA, MPO and SOD. Result Compared with the AP group, the serum content of IL-6, IL-β and TNF-α were significantly decrease in RPDP group（P 〈 0.05） , the content of MDA and MPO in pancreatic tissue in every time quantum were also decrease in RPDP group（P 〈0. 05）, while the content of SOD were significantly improve（P 〈0.05）. Conclusion Dahuang Mudan Decoction could repress the release of TNF-α, IL-6 and IL-β, could decrease the product of MDA, and had the effect of anti-oxidative damage, cleaning oxygen free radicals.

关 键 词：大黄牡丹汤组方 急性胰腺炎 炎症介质 氧自由基 

分 类 号：Q95-33[生物学—动物学]