HPLC法测定苯扎溴铵器械消毒液中苯扎溴铵含量  

Determination of Benzhalkonium Bromide in Benzhalkonium Bromide Solution for Disinfection of Instruments by HPLC

作　　者：鞠蕾[1] 陈珊珊[1] 孙旭婧 徐元元[1] 付桂英[1] 

机构地区：[1]解放军307医院,北京100071

出　　处：《解放军药学学报》2014年第5期444-446,共3页Pharmaceutical Journal of Chinese People's Liberation Army

基　　金：2013年度军队医疗机构制剂标准提高计划科研专项课题;No.13ZJZ04-1

摘　　要：目的建立苯扎溴铵器械消毒液中苯扎溴铵的含量测定方法。方法采用HPLC法测定苯扎溴铵含量,Agilent SB-C18色谱柱（4.6 mm×250 mm,5μm）,流动相为0.07 mol·l^-1乙酸铵（内含1%三乙胺）-乙腈（30∶70）〔醋酸调pH（3.0±0.1）〕,检测波长为262 nm,流速为1.0 ml·min^-1,进样量为10μl。结果苯扎溴铵在浓度为600~1600μg·ml^-1范围内与峰面积呈良好的线性关系,r=0.9999,平均回收率为100.0%,RSD为1.06%。结论 HPLC法简单准确、样品无需处理,可以用于苯扎溴铵器械消毒液的含量测定。Objective To establish a determination method of benzhalkonium bromide solution for disinfec- tion of instruments. Methods HPLC was used to determine the content of benzhalkonium bromide solution for disin- fection of instruments. An Agilent SB-C18 column （4.6 mm×250 mm,5μm） was used with a mobile phase com- posed of 0.07 mol-L^-1 ammonium acetate solution （1% triethylamine）-acetonitrile （30： 70）. The pH value was adjusted to （3.0 ± 0.1 ） by glacial acetic acid. The detection wavelength was 262 nm, the flow rate 1.0 ml. min^- 1, and the injection volume 10 μl. Results Within the range of 600 -1600 ±g .ml^-1, the concentration of benzalkonium bromide showed a good linear relationship within the peak area （ r = 0. 9999）. The average recovery was 100.0% with an RSD of 1.06%. Conclusion The established method is simple, specific, reliable and reproduci- ble,which can be used for quality control of benzhalkonium bromide solution for disinfection of instruments.

关 键 词：苯扎溴铵 HPLC 

分 类 号：R927.2[医药卫生—药学]