大口黑鲈胰腺的组织学观察  

The histological observation of the pancreas of largemouth bass,Micropterus salmoides

作　　者：佟春萌[1] 陈乃松[1] 季振尧[1] 徐祥泰 苟仕潘 

机构地区：[1]上海海洋大学水产与生命学院,上海201306

出　　处：《上海海洋大学学报》2014年第6期814-819,共6页Journal of Shanghai Ocean University

基　　金：上海市科学技术委员会项目(10320503100);上海高校知识服务平台(ZF1206)

摘　　要：采用组织切片和免疫组化技术对大口黑鲈的胰腺进行了组织学观察。经H-E染色和G-醛复红染色了的切片显示:大口黑鲈腹腔中10多个肉眼可见的黄白色颗粒物其实是被一层结缔组织和外分泌胰腺包裹着的胰岛结构,其中最大的一个即为主岛;还有许多肉眼不可见的胰岛结构被埋藏于总胆管的管壁中;肝脏中有外分泌胰腺的存在与分布。过氧化物酶标记的链霉卵白素免疫组化切片和图像分析显示:B细胞主要集中胰岛的中央区;A细胞分布于胰岛的全部。本研究的结论为:大口黑鲈的内分泌胰腺主要分布于总胆管周围,外分泌胰腺除了包裹于胰岛之外也分布于肝脏中,胰腺的分布属于弥散型。In the present study,the techniques of histological section and immunohistochemistry were adopted to perform a histological observation on the pancreas of largemouth bass. It was revealed by the sections stained with H-E and G-aldehyde fuchsine that more than 10 yellow-white macroscopic nodules were identified as islet structures in the abdominal cavity of largemouth bass,in which the biggest one was designated as principal islet,and a lot of smaller invisible islets were embedded in the wall of the common bile duct. The islet was surrounded by an inner layer of connective tissue and an outer layer of exocrine pancreatic tissue,and the exocrine pancreatic tissue also existed and was distributed in the liver. The sections marked by streptavidin peroxidase（ S-P） immunohistochemical method and the section image analysis suggested that B cells were located mainly in the central region of the islet while the A cells were distributed throughout the islet. It can be concluded that the pancreas of largemouth bass belongs to dispersal type,the endocrine pancreas（ islet） is distributed mainly around the common bile duct while the exocrine pancreas surrounds the islet and it is also embedded in the liver.

关 键 词：大口黑鲈 胰腺 胰岛 组织学观察 

分 类 号：S917[农业科学—水产科学]