盐度梯度介导mTOR信号通路对尼罗罗非鱼生长的影响  

Effects of salinity on growth of Oreochromis niloticus through m TOR signal pathway

作　　者：王飞[1] 庄青青[1] 梁从飞[1] 颜标[1] 赵金良[1] 

机构地区：[1]上海海洋大学农业部淡水水产种质资源重点实验室,上海201306

出　　处：《上海海洋大学学报》2014年第6期820-825,共6页Journal of Shanghai Ocean University

基　　金：现代农业产业技术体系专项资金(CARS-49-4B);上海高校知识服务平台上海海洋大学水产动物遗传育种中心(ZF1206)

摘　　要：mTOR信号通路是动物生长发育的重要调控通路之一。以尼罗罗非鱼为实验材料,研究了不同盐度梯度对尼罗罗非鱼生长的影响,以及mTOR通路下游调节因子p70s6k和4ebp1的mRNA与蛋白表达水平变化。30 d养殖结果显示,盐度增加对尼罗罗非鱼生长具有明显抑制作用,0、15、20、25盐度下,尼罗罗非鱼日均增重率分别为(0.48±0.13)g/d、(0.31±0.09)g/d、(0.14±0.08)g/d、(0.09±0.03)g/d。荧光定量PCR显示,p70s6k mRNA相对表达量随着盐度梯度升高而下降,0盐度组中表达量最高,显著高于其他盐度组(P<0.05),25盐度组最低;4ebp1 mRNA相对表达量随着盐度梯度升高而升高,0盐度组中表达量最低,25盐度组最高,0盐度组与25盐度组间差异显著(P<0.05)。Western blot结果显示,0盐度组中p70s6k蛋白含量显著大于其他高盐度组,而25盐度组中4ebp1蛋白含量显著大于其余低盐度组(P<0.05)。结果表明,盐度可能通过介导mT OR信号通路参与尼罗罗非鱼的生长调节。m TOR signal pathway plays a vital role in the regulation of animal growth and development. In this study,growth rates of Oreochromis niloticus at different salinities were compared in a 30-day experiment; in the meantime,expression changes of downstream pathway regulation factors p70s6 k and 4ebp1 were evaluated at both mRNA and protein levels. The results showed that salinity significantly inhibited the growth of Nile tilapia,the final body weight decreased with the salinity increase,and average body weight at 0,15,20,25 salinity was（ 0. 48 ± 0. 13） g / d,（ 0. 31 ± 0. 09） g / d,（ 0. 14 ± 0. 08） g / d,（ 0. 09 ± 0. 03） g / d,respectively.The p70s6 k mRNA expression level decreased with salinity increasing,the highest was detected at 0 salinity,while the lowest was at 25 salinity,which was significantly lower than that at 0,15 and 20 salinities. The4ebp1 mRNA expression at 25 salinity was significantly higher than 0,15,20 salinity,increasing with salinity levels. Western blot results showed the protein level of p70s6 k at 0 salinity was significantly higher than that at other higher salinities,while 4ebp1 at 25 salinity was significantly higher than that at other lower salinities.Our result suggested salinity may affect growth of O. niloticus through m TOR signaling pathway.

关 键 词：尼罗罗非鱼 盐度 生长 P70S6K 4ebp1 

分 类 号：S917[农业科学—水产科学]