马钱子碱氮氧化物对MKN-45瘤株增殖及活体血管生成的影响  

Inhibiting Effect of Brucine N-Oxide on the Proliferation of MKN-45 Cancer Cell and Angiogenesis of Egg Embryo

作　　者：潘稚云[1] 朱天冀 贾茹[1] 潘扬[1] 

机构地区：[1]南京中医药大学药学院,江苏南京210023

出　　处：《南京中医药大学学报》2014年第6期557-560,共4页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：江苏高校优势学科建设工程资助项目(ysxk-2010);南京中医药大学科技重点培育项目(09XPY03)

摘　　要：目的 考察马钱子碱氮氧化物（Brucine N-oxide,BNO）对MKN-45瘤株增殖及活体血管生成的影响.方法 通过MTT比色法检测BNO对MKN-45细胞增殖的抑制作用;并采用鸡胚绒毛尿囊膜（CAM）模型,以不同浓度BNO给药,解剖显微镜下观察BNO对CAM血管形态的影响,同时对新生血管数目进行计数并计算抑制率.结果 ①BNO在50～350μmol/L剂量范围内可以剂量依赖性地抑制MKN-45胃腺癌细胞增殖;②BNO用药后CAM血管数目明显减少,出现血管形态异常甚至无血管区;与对照组相比,不同浓度的BNO对CAM血管形成均呈现一定的抑制作用,且有极显著的剂量依赖性（r2=0.901 0,P＜0.01）;在5～15 mmol/L范围内可显著抑制血管的生成（P＜0.05～0.01）,最高抑制率达26％.结论 BNO具有一定的抑癌及抗活体血管生成的作用,提示其对肿瘤或其他病理情况下的抗血管治疗具有重要应用价值.OBJECTIVE To investigate the effect of brucine N-oxide （BNO） on the proliferation of MKN-45 cancer cell and angiogenesis of egg embryo.METHODS The proliferation of MKN-45 cell was detected by MTT method.The chicken chorioallantoic membrane （CAM） assay was used to detect the effect of BNO on angiogenesis in vivo.RESULTS The inhibiting effect of BNO on the proliferation of MKN-45 cancer cell was dose dependent in the dose range of 50～350 μmol/L.The number of micro vessels of CAM decreased after the treatment of BNO,and there were also some abnormal-looking blood vessels and avascular zones around the site of drug application.Different concentration of BNO all had inhibitory effects on angiogenesis with concentration dependency （r2 =0.901 0,P〈0.01）.Compared with controls,BNO inhibited significantly the angiogenesis in CAM in the dose range of 5～ 15 mmol/L （P〈0.05 ～0.01）,with the maximum inhibiting rate of 26 %.CONCLUSION BNO has certain anti-cancer effect and can inhibit blood vessel formation in CAM,which implies its worth in treatment on angiogenesis in tumor or other pathological conditions.

关 键 词：血管生成 马钱子碱氮氧化物 MKN-45瘤株 鸡胚绒毛尿囊膜 

分 类 号：R285.5[医药卫生—中药学]