He-Ne激光对啤酒大麦麦芽同工酶基因表达的影响  

Effects of He-Ne Laser on the Isozyme Expression of Beer Barley

作　　者：闫蓉[1] 虞波[1] 李娜[1] 薛燕燕[1] 赵岩[1] 韩榕[1] 杨利艳[1] 

机构地区：[1]山西师范大学生命科学学院,山西临汾041004

出　　处：《激光生物学报》2014年第5期417-422,共6页Acta Laser Biology Sinica

基　　金：山西师范大学大学生创新创业训练项目(SD2013CXCY-76)资助课题

摘　　要：采用不同时间He-Ne激光(5 m W/mm2)对"临啤二号"大麦种子进行辐照,利用聚丙烯酰胺凝胶电泳(PAGE)技术分析激光辐照对大麦麦芽中超氧化物歧化酶(SOD)同工酶、过氧化物(POD)同工酶、过氧化氢(CAT)同工酶、β-淀粉酶同工酶、三磷酸腺苷(ATPase)同工酶酶谱的影响。结果表明:种子经He-Ne激光辐照后诱导出1条新POD同工酶谱带,且总POD活性在60 s和90 s处有所增加;30 s激光辐照诱导出1条新的CAT同工酶谱带,其它时间处理酶的组分没有变化,但酶活性增加;He-Ne激光辐照使SOD、β-淀粉酶及ATPase同工酶的组分在不同时间下有所减少,但一定时间的激光辐照提高了其同工酶的活性。综合实验结果可知,60 s^90 s He-Ne激光可以促进大麦种子萌发过程中以上同工酶基因的表达,有利于缩短制麦周期,提高麦芽糖化力及抗氧化力。The seeds of a beer barley cultivar（Linpi No. 2） were exposed to He-Ne laser irradiation（5 m W / mm2）with varied duration,the polyacrylamide gel electrophoresis（PAGE） was used to analyze the patters of superoxide dismutase（SOD）,peroxidase（POD）,catalase（CAT）,β-amylase and adenosine triphosphatase（ATPase） in malt. The results showed that one new band of POD zymogram appeared and the activities of total POD enhanced in 60 s and 90 s radiation groups after He-Ne laser irradiation compared with the control; as for CAT,it showed that one new band of CAT zymogram appeared in 30 s radiation group and the activities of total CAT enhanced though there are no isozyme bands changing in other groups; there were isozyme bands disappearing in SOD,β-amylase and ATPase in different radiation groups compared to that of control,but the activities of the isozymes increased after certain duration radiation. To sum up,60 s ~ 90 s irradiation promoted the expression of above isozyme genes which might shorten the malting process and improve the malt diastatic power and antioxidant capacity.

关 键 词：大麦 同工酶 HE-NE激光 基因表达 

分 类 号：Q631[生物学—生物物理学] Q691.5