鲜、干马齿苋药材中生物碱类成分的含量差异  

Content Difference of Total Alkaloids in Fresh and Dried Portulacae Herba

作　　者：杨成俊[1] 王理想[2] 封亮[2] 

机构地区：[1]江苏联合职业技术学院连云港中医药分院,江苏连云港222007 [2]江苏省中医药研究院,南京210028

出　　处：《中国实验方剂学杂志》2014年第24期88-90,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：江苏省中医药领军人才项目(2006)

摘　　要：目的：比较鲜、干药材中去甲肾上腺素和多巴胺的含量差异。方法：采用HPLC-UV测定马齿苋中去甲肾上腺素和多巴胺含量,流动相甲醇-水[70∶30,含30 mmol·L^-1十二烷基硫酸钠和0.02 mol·L^-1磷酸盐缓冲液（p H 3.2）],流速0.8 m L·min-1,检测波长280 nm。结果：HPLC中去甲肾上腺素和多巴胺的分离度很好,二者线性范围分别为52.5~157.5,64.7~194.1 mg·L^-1。鲜马齿苋中去甲肾上腺素和多巴胺质量分数分别为0.070%和0.50%,二者在干品中质量分数分别为0.015%和0.20%。结论：鲜药材中去甲肾上腺素和多巴胺含量明显高于干药材,可为马齿苋的合理使用提供实验依据。Objective：To compare contents of norepinephrine and dopamine in fresh and dry Portulacae Herba.Method：HPLC-UV method was adopted for determination of norepinephrine and dopamine in fresh and dry Portulacae Herba.This analysis was performed on a Alltima C18 column （4.6 mm × 250 mm,5 μm）,column temperature was set at 25 ℃,flow rate was 0.8 mL ·min-1,detection wavelength was kept at 280 nm,mobile phase consisted of methanol-water （70∶ 30,containing 30 mmol ·L^-1 sodium dodecyl sulfate and 0.02 mol ·L^-1 phosphate buffer with pH of 3.2）.Result：Separation of norepinephrine and dopamine was well in HPLC,these two alkaloids showed a good linear relationship in 52.5-157.5 mg ·L^-1 and 64.7-194.1 mg ·L^-1,respectively.Contents of norepinephrine and dopamine in fresh Portulacae Herba were 0.070% and 0.50% ; while contents of them in dry Portulacae Herba were 0.015% and 0.20%,respectively.Conclusion：Contents of norepinephrine and dopaminein in fresh herbs are significantly higher than these in dry herbs.This paper provides experimental basis for reasonable application of Portulacae Herba.

关 键 词：马齿苋 鲜、干药材 去甲肾上腺素 多巴胺 

分 类 号：R283.1[医药卫生—中药学] R284.1[医药卫生—中医学]