胃元宁对功能性消化不良小鼠胃肠运动及胃肠激素分泌的影响  

Effects of Weiyuanning on Gastrointestinal Motility and Gastrointestinal Hormones in Mice with Functional Dyspepsia

作　　者：吴洋[1,2] 马学琴[1,2] 王洋 王继永 

机构地区：[1]宁夏医科大学药学院,银川750004 [2]宁夏医科大学回族药现代化工程技术研究中心,银川750004 [3]宁夏银川市口腔医院,银川750004 [4]宁夏银川市中医院,银川750004

出　　处：《中国实验方剂学杂志》2014年第24期185-189,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：宁夏回族自治区医疗卫生科研计划项目(XM2012046)

摘　　要：目的:建立功能性消化不良(functional dyspepsia,FD)小鼠模型,研究胃元宁对FD小鼠胃肠运动及胃肠激素分泌的影响。方法:昆明种小鼠随机分为6组为正常组,胃元宁汤剂高、中、低剂量组(90,45,22.5 g·kg-1),模型组,多潘立酮组(10 mg·kg-1),除正常组外,其他小鼠采用不规律进食联合左旋精氨酸(L-arginine,L-Arg)ip构建FD小鼠模型,造模成功后ig给予相应药物7 d,检测小鼠体重、胃内残留率和肠推进率;观测胃组织病理学变化;利用酶联免疫吸附测定法(ELISA)检测血清胃泌素(gastrin,GAS),血浆胃动素(motilin,MTL)的含量变化。结果:与正常组相比,各造模组小鼠体重及小肠推进率均明显下降(P<0.01),胃内残留率均显著升高(P<0.01);经治疗后,与模型组相比,胃元宁各剂量组均能显著增加小鼠体重及小肠推进率,并显著升高血清GAS、血浆MTL含量(P<0.05,P<0.01),同时可明显降低胃内残留率(P<0.05,P<0.01)。治疗前后,与正常组相比,各实验组胃组织病理学表现均无差异。结论:不规律进食联合左旋精氨酸腹腔注射可成功构建FD小鼠模型;胃元宁能够促进胃肠运动,显著改善小鼠功能性消化不良,其机制可能与其升高血清GAS和血浆MLT水平有关。Objective：To observe the effects of Tongbian granules on the regulation of aquaporin 3 （AQP3） and aquaporin 8 （AQP8） in rats with slow transit constipation （STC）,and to clarify its possible mechanism.Method：A slow constipation rat mode was induced by compound phenylethyl piperidine.The 50 model rats were randomly divided into model group,low-,medium-and high-dose Tongbian granules groups （9.3,18.7,37.3 g ·kg^-1）,and polyethylene glycol powder group （2.1 g ·kg^-1）.The another 10 normal rats were assigned to normal control group.All rats were ig adiminstrated with the corresponding medicines for 30 days.The body weight,24-hour stool weight,the carbon powder propulsion in the colon of rats were recorded and measured.The distribution and relative content of AQP3 and AQP8 in rat colon were detected.Result：Compared with model group,the body weight,24-hour stool weight,the carbon powder propulsion rate in the colon were decreased in model group.The above indexes were improved in the Tongbian granules groups （P 〈 0.05）.Immunohistochemistry results showed that the number of AQP3 and AQP8 were remarkably decreased in all dose Tongbian granules groups and polyethylene glycol powder group.Tongbian granules group of high dose had the best results （P 〈 0.05）.Conclusion：Tongbian granules has excellent curative effects in improving constipation defecation function of STC rats,which may be involved with the regulation of absorption and secretion of moisture achieved by depressing AQP3 and AQP8 in rat colon.

关 键 词：胃元宁 功能性消化不良 胃肠运动 胃泌素 胃动素 

分 类 号：R285.5[医药卫生—中药学]