高效液相色谱法测定枣叶中酸枣仁皂苷B的含量  

Determination of jujuboside B in jujube leaves by HPLC

作　　者：李喜悦[1] 高哲[1] 安小楠 崔璨[1] 王荣芳[1] 崔同[1] 

机构地区：[1]河北农业大学食品科技学院,保定071000

出　　处：《食品科技》2014年第12期299-302,共4页Food Science and Technology

基　　金：国家林业公益性行业科研专项(201304708)

摘　　要：建立了一种测定枣叶中酸枣仁皂苷B含量的高效液相色谱分析方法,色谱条件为：色谱柱Hypersil BDS C18（4.6 mm×200 mm,5μm）;流动相为乙腈：水（35：65,v/v）,加入甲酸0.05%;流速为0.8 mL/min;柱温30℃;检测波长204 nm。结果表明：酸枣仁皂苷B在20-1000μg/m L范围内具有良好的线性关系（r2=0.9999）,平均加标回收率为100.1%,最低检出限1.1μg/mL。采用开发的高效液相色谱法对14个品种的大枣及酸枣的幼叶中酸枣仁皂苷B的含量进行了测定,其含量在0.91-2.67 mg/g之间。The purpose was to establish a method for determining jujuboside B in jujube leaves using HPLC. The samples were separated on Hypersil BDS C18 column（4.6 mm×250 mm, 5 μm）, the mobile phase consisted acetonitrile：water（35：65, v/v） and 0.05% formic acid, the flow rate was 0.8 mL/min, the detection wavelength was 204 nm. The developed method exhibited good linearity over the range from 20-1000 μg /mL with a correlation of 0.9999. The average recovery rate for jujuboside B was 100.1%, the minimum detection limit was 1.1 μg/m L. By this method we analysed young jujube leaves from 14 different varieties, the content of jujuboside B was between 0.91 and 2.67 mg/g.

关 键 词：枣叶 酸枣仁皂苷B 高效液相色谱(HPLC) 

分 类 号：O657.72[理学—分析化学]