短期能量限制对正常大鼠胰岛素敏感性的影响  

Effects of short-term caloric restriction on insulin sensitivity in normal rats

作　　者：朴雄[1] 卢焕俊 李香兰[2] 姜海英[2] 

机构地区：[1]延边大学附属医院,吉林延吉133002 [2]延边大学医学院

出　　处：《山东医药》2014年第38期29-31,共3页Shandong Medical Journal

基　　金：吉林省卫生厅科研计划项目(2013Z071)

摘　　要：目的探讨短期能量限制(CR)对正常大鼠胰岛素敏感性的影响及其机制。方法将24只F344/NSIc系雄性大鼠随机分为对照组(AL组)和能量限制组(CR组)各12只。AL组自由摄入食物及水,CR组限制食物摄入(不禁水)8周(饲料总摄入量为对照组的64%)。采用高胰岛素—正常血糖钳夹试验测定两组葡萄糖输注率(GIR)判断其胰岛素敏感性(GIR越高,胰岛素敏感性越高);比较两组体质量增加及内脏脂肪重量;Western blot法检测骨骼肌葡萄糖转运体4(Glu T4)、蛋白激酶B底物蛋白160(AS160)及磷酸化AS160(p-AS160)蛋白表达。结果CR组GIR明显高于对照组,体质量及内脏脂肪重量明显低于对照组;两组Glu T4、AS160及p-AS160蛋白表达无显著差异。结论短期CR可提高大鼠正常状态下的胰岛素敏感性,其机制可能与AS160上游的胰岛素信号转导蛋白有关。Objective To investigate the effect of the short-term caloric restriction（ CR） on insulin sensitivity and its signal transduction mechanisms. Methods A total of 24 F344 / NSIc rats were equally divided into ad libitum group（ AL group） and CR group. Rats in AL group were free fed every alternate day for 8 weeks,while rats in CR group were fed restrict caloric without water deprivation for 8 weeks. Glucose infusion rate（ GIR） and insulin sensitivity were measured by using hyperinsulinemic-euglycemic clamp method. The body weight mass and visceral fat weight between the two groups were compared. The muscle glucose transporter 4（ Glu T4）,kinase substrate 160（ AS160） protein and p-AS160 protein was detected by using Western blot method. Results GIR in CR group was obviously higher than that of control group,while body mass and visceral fat weight significantly lower than control group. However,there was no significant difference in expression of Glu T4,AS160 protein and p-AS160 protein. Conclusions Short-term caloric restriction can improve the insulin sensitivity in healthy rats which may be associated with reduced the visceral fat increasing.

关 键 词：能量限制 胰岛素敏感性 葡萄糖转运体4 蛋白激酶B底物蛋白160 

分 类 号：R587.1[医药卫生—内分泌]