七氟烷预处理联合后处理对肺缺血再灌注损伤大鼠血浆ET-1及肺组织TNF-α的影响  

Effect of sevoflurane preconditioning combined with postconditioning on ET-1 and TNF-α expression in rats during lung ischemia-reperfusion

作　　者：杜宁[1] 徐桂萍[2] 刘备[1] 

机构地区：[1]石河子大学医学院,新疆石河子832000 [2]新疆维吾尔自治区人民医院

出　　处：《山东医药》2014年第42期1-3,7,共4页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(81160016)

摘　　要：目的探讨七氟烷预处理联合后处理对肺缺血再灌注(I/R)损伤大鼠血浆内皮素-1(ET-1)及肺组织TNF-α表达的影响。方法 SD大鼠54只,随机分为假手术组(S组)、肺I/R组、七氟烷预处理与后处理联合组(SPr+o组),每组各18只。I/R组、Spr+o组采用改良的Epinger方法制备肺I/R模型。S组仅游离大鼠左肺门,但不阻断。Spr+o组阻断左肺门前给予2.1%七氟烷吸入30 min,洗脱10 min;阻断左肺门45 min后,在恢复灌注的同时给予2.1%七氟烷吸入30 min,之后再继续灌注90 min。S组及I/R组不给予七氟烷干预。于再灌注30、60和120 min时各组随机取6只大鼠,处死取其肺组织测湿干重(W/D)比,ELISA法测定血浆ET-1及肺组织TNF-α水平,HE染色观察肺组织病理改变。结果与S组相比,Spr+o组与I/R组再灌注各时点肺组织W/D、TNF-α及血浆ET-1水平均升高(P<0.05)。与I/R组相比,Spr+o组再灌注各时点肺组织W/D、TNF-α及血浆ET-1水平均下降(P<0.05)。Spr+o组再灌注各时点肺组织病理损伤与I/R组相比均有所减轻。结论七氟烷预处理联合后处理可减轻肺I/R损伤,其机制可能与抑制血浆ET-1水平及肺组织TNF-α表达有关。Objective To investigate the effects of sevoflurane preconditioning combined with postconditioning on expression of plasma ET-1 and lung tissue TNF-α in rats during lung ischemia-reperfusion. Methods Fifty-four SD rats were randomly divided into three groups,which were sham group（ S group）,lung ischemia-reperfusion group（ I / R group）,sevoflurane pretreatment and post-treatment combination group（ Spr ＋ o group）,with 18 rats in each group. Rats in I / R group,Spr ＋ o group were made I / R models by using the modified Epinger lung ischemia-reperfusion method. Rats in S group was only isolated pulmonary hilum but not ligated. Rats in Spr ＋ o group were inhaled 2. 1% sevoflurane for 30 minutes before ischemia,then the left pulmonary hilum were clamped for 45 minutes and were inhaled 2. 1% sevoflurane for 30 minutes followed a 90 minutes reperfusion. S group and I / R group were not given sevoflurane intervention. At 30,60 and 120 minutes reperfusion,six rats in each group were randomly selected to sacrifice in order to measure the lung tissue wet / dry（ W / D）weight ratio. ELISA assay was used to test the plasma ET-1 and lung tissue TNF-α levels. Pathological changes in the lung tissue were observed by using HE staining. Results Compared with the S group,lung tissue W / D,TNF-α and plasma ET-1 levels of Spr ＋ o group and I / R group were increased at each time point reperfusion（ P〈0. 05）. Compared with the I / R group,lung tissue W / D,TNF-α and plasma ET-1 levels of Spr ＋ o group were decreased at each time point reperfusion（ P〈0. 05）. Microscopic examination showed that the pathologic changes were significantly attenuated in Spr ＋ o group compared with I / R group. Conclusions Sevoflurane preconditioning combined with postconditioning can reduce lung ischemia-reperfusion injury,which may be related to the inhibition of plasma ET-1 and lung TNF-α expression.

关 键 词：七氟烷 预处理 后处理 再灌注损伤 肺 内皮素-1 肿瘤坏死因子-α 

分 类 号：R655.3[医药卫生—外科学]