重穗型水稻穗部性状及剑叶宽的QTL定位  

QTL Mapping of Flag Leaf Width and Panicle Traits of Heavy-panicle Rice

作　　者：张向阳[1] 张红宇[1] 徐培洲[1] 陈晓琼[1] 田永航[1] 王志 黄廷友 吴先军[1] 

机构地区：[1]四川农业大学水稻研究所/西南作物基因资源与遗传改良教育部重点实验室,四川成都611130 [2]绵阳市农业科学研究院,四川绵阳621000

出　　处：《杂交水稻》2014年第6期56-61,共6页Hybrid Rice

基　　金：公益性行业科研专项"长江上游稻区超级杂交稻高效育种技术与新品种选育"(201403002-3)

摘　　要：利用穗部性状存在显著差异的籼稻材料千粒稻与粳稻材料日本晴为亲本,构建F2作图群体,采用复合区间作图法,以筛选出具多态性的136个分子标记对每穗总粒数、一次枝梗数、二次枝梗数、穗长、剑叶宽、着粒密度6个性状进行QTL检测。共检测到20个QTL,分别位于水稻第1,2,3,5,7,8,9和10号染色体上,单个QTL对相关表型变异的贡献率为3%~18%,LOD值范围在2.55~6.13,其中效应值≥10%的位点有13个,≤5%的位点检测到5个。相关性状QTL有成簇分布在染色体相同或者相近区域的趋势。Using 136 selected polymorphic DNA markers （92 SSR and 44 Indel）, the QTL mapping was conducted on flag leaf width （Flw） and five panicle traits, i. e. , the number of spikelets per panicle or grain number （ Gn）, primary branch number per panicle （Pbn）, secondary branch number per panicle （Sbn）, panicle length （P1） and spikelet density （Sd）, by composite interval mapping based on an F2 population from the cross between indica Qianlidao and japonica Nipponbare （ NIP）, two parents with distinct panicle traits. The results showed that 20 QTLs of the target traits were detected on Chromosomes 1,2, 3, 5, 7, 8, 9 and 10. The contribution to phenotyp- ic variation of a single QTL ranged from 3% to 18% with LOD scores between 2.55 and 6.13. Among all of the QTLs, 13 had a genetic effect equal to or greater than 10% and five less than or equal to 5%. Furthermore, there existed a tendency of cluster distribution on the chromosomes for the QTLs of those closely related traits in pheno- type.

关 键 词：重穗型水稻 穗部性状 遗传图谱 QTL定位 

分 类 号：S511.01[农业科学—作物学] Q78[生物学—分子生物学]