补肾益气法中药预防用药对膝骨性关节炎家兔血清中AQP1及AQP3表达的影响  

Effects of Chinese Medicine Replenishing Kidney and Invigorating Qi on Expression of AQP1 and AQP3 in Knee Osteoarthritis of the Rabbit Serum

作　　者：康武林[1] 袁普卫[2,3] 张根印[2,3] 刘德玉[2,3] 

机构地区：[1]陕西中医学院,陕西咸阳712000 [2]陕西中医学院附属医院,陕西咸阳712000 [3]陕西中医学院骨伤研究所,陕西咸阳712000

出　　处：《四川中医》2014年第11期32-35,共4页Journal of Sichuan of Traditional Chinese Medicine

基　　金：陕西省重点科技创新团队项目(编号:2013KCT-26);陕西省自然科学基础研究计划项目(编号::2010JM4002);陕西省科学技术研究发展计划项目(编号:2011kjxx33);陕西省科学技术研究发展计划项目(编号:2009K01~63);陕西省中医管理局中医药科研课题(编号:jc25);陕西省中医管理局中医药科研课题(编号:13-LC054);陕西省教育厅重点学科及国家中医管理局重点学科专项基金资助

摘　　要：目的：探讨补肾益气法中药预防家兔膝骨性关节炎的机制。方法：4月龄健康日本长耳大白兔,72只,雄性,体重为（1.8~3.5）kg,随机分为六组,空白组（A组）、模型组（B组）、中药高剂量组（C组）、中药中剂量组（D组）、中药小剂量组（E组）、盐酸氨基葡萄糖胶囊对照组（F组）,每组12只。除A组外,其余均参考Hulth造模方法造兔膝骨性关节炎模型,在造模同时C、D、E、F组分别给予不同剂量补肾益气中药及盐酸氨基葡萄糖胶囊灌胃4周,以行预防治疗,造模6周后均采用耳中央动脉取血,应用ELISA法检测血清中AQP1及AQP3的含量,同时取关节软骨行大体及光镜检查。结果：C、D、E、F组与B组血清中AQP1及AQP3含量比较均有显著性差异（P〈0.05）,C组、A组与B组比较有显著性差异（P〈0.05）,补肾益气中药中剂量组与盐酸氨基葡萄糖胶囊组无明显差异（P〉0.05）,补肾益气中药高、中、低剂量组之间比较有著性差异（P〈0.05）。组织学显示预防治疗各组动物关节软骨有不同程度的色泽变化,骨赘、骨囊肿形成,软骨关节面出现浅表性糜烂,部分标本软骨缺损深达软骨中层,出现软骨剥脱,但程度明显低于模型组。结论：补肾益气法中药可能通过降低兔血清中AQP1及AQP3含量,来达到预防骨性关节炎的发生。Objective： To probe into the mechanism of the Chinese herbs with functions of reinforcing kidney and supplementing Qi for preventing knee osteoarthritis of the rabbit. Methods： Totally 72healthy Japan long-ear white rabbits, aged 4months, were randomly divided into 6groups, blank group （A）, model group （B）, high dose Chinese herb group （C）, middle dose Chinese herb group （ D）, small dose Chinese herb group （ E）, Aminoglucose Hydrochloride Capsule control group （F）, 12rabbits in each group. All the rabbits in the groups, except the group A, the others all reference Hulth made mould method the rabbit model of knee osteoarthritis. At the same time of modeling, the different doses of Juanbi Capsules and Aminoglucose Hydrochloride Capsule were administrated intragastrically in the group C, D, E, F respectively, for 4weeks, for preventive treatment. In the group B, the rabbit was administrated intragastrically with equal volume of normal saline to the medication groups, twice each day, in the morning and the evening, and in the group A, nothing was administrated. After modeling for 6weeks, the joint fluid was taken and AQPland AQP3contents were detected with ELISA method, and the articular cartilage was taken for macroscopic and microscopic examinations. Results： In all the preventive treatment groups, the articular cartilage color changed to varying degrees with formation of osteophyte and bone cyst, superficial erosion on the chondral articular surface, and the cartilage defect reached to the mid layer in a part of specimens with cartilage exfoliation, but which in the extent were sig- nificantly lower than those in the model group. There were significant differences between the group A and B in AQP1 and AQP3contents in the joint fluid （P〈0. 05）, indicating that the modeling was successful; and there were significant differences as group B compared with the group C, D, E, F, showing that TNF-α, IL-land IL-6contents were decreased in all the medica-tior, groups ; and significant di

关 键 词：补肾益气法 骨性关节炎 预防 AQP1 AQP3 

分 类 号：R285.5[医药卫生—中药学]