基于氧化石墨烯的Ag^+与核酸中C-C碱基错配的相互作用研究  

Study on the interaction between Ag+ion and C-C mismatched base pair based on graphene oxide

作　　者：吴呈珂[1] 岳园园[1] 郑立庆[2] 安晓馀[1] 冯素玲[1] 

机构地区：[1]河南师范大学化学化工学院,新乡453007 [2]河南师范大学环境学院,新乡453007

出　　处：《分析试验室》2014年第12期1370-1373,共4页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(21205029);河南省基础与前沿技术研究计划项目(122300410286)资助

摘　　要：基于氧化石墨烯,以修饰了荧光分子的单链DNA为探针,利用荧光光谱和圆二色光谱研究了Ag+对双螺旋DNA中C-C碱基错配特异性识别,并建立了检测Ag+的荧光方法。荧光光谱表明Ag+能与带C-C错配碱基的双螺旋DNA作用,使原本被氧化石墨烯淬灭的标记在DNA上的荧光分子的荧光得到恢复。圆二色光谱表明Ag+能与双螺旋DNA中的C-C碱基错配相互作用,形成更稳定的C-Ag+-C结构。在最佳实验条件下,体系荧光强度的变化与Ag+的浓度在30.0~250.0 nmol/L之间呈良好的线性关系,方法检出限为19.1 nmol/L。为研究Ag+与核酸分子的相互作用及其在环境中对生物分子的影响等方面提供了重要依据。Based on graphene oxide and using a fluorescent molecule modified single-stranded DNA（ ss DNA） as a probe,the specific binding between Ag^＋ion and C-C mismatched base pair in duplex DNA has been studied by fluorescence spectroscopy and circular dichroism spectra. Moreover,a fluorescent method for detecting Ag^＋ion has been established. Fluorescence spectroscopy showed Ag^＋ion could stabilize the C-C mismatched base pair in double-stranded DNA,and the fluorescence of fluorescent marker on the DNA which was quenched by graphene oxide without Ag^＋ions was restored. Circular dichroism spectra showed that Ag＋ion could specifically bind with the C-C base pair in the double-stranded DNA,and formed a more stable C-Ag^＋-C structure. Under the optimum conditions,the change of fluorescent intensity has good linear relationship with the concentration of Ag^＋ions between 30. 0 - 250. 0 nmol / L,and the detection limit was 19. 1 nmol / L. This method provides an important adminicle on the interaction between Ag^＋ions and nucleic acid molecules,and biological molecules affected by Ag＋ions in the environment.

关 键 词：氧化石墨烯 AG^+ DNA 

分 类 号：O657.3[理学—分析化学]