LC-MS/MS法同时测定祛痘类化妆品中林可霉素等20种禁用物质  

Simultaneous determination of lincomycin and other 19 prohibited substances in anti-acne cosmetics by LC-MS / MS

作　　者：简龙海[1] 郑荣[1] 钟吉强[1] 陈静[1] 孟茜[1] 王柯[1] 

机构地区：[1]上海市食品药品检验所,上海201203

出　　处：《分析试验室》2014年第12期1459-1462,共4页Chinese Journal of Analysis Laboratory

摘　　要：建立了祛痘类化妆品中林可酰胺类、四环素类、硝基呋喃类抗生素及氯霉素、甲硝唑等20种禁用物质的LC-MS/MS同时测定法。样品以甲醇-1%甲酸溶液（1∶1,V/V）进行提取。色谱柱为ZORBAX SB-C18（2.1 mm×150 mm,3.5μm）。质谱仪采用电喷雾离子化源、正离子多反应监测模式。20种待测物在10-250 ng/m L范围内线性关系良好（r〉0.995）;检出限在0.01-0.2 mg/kg之间;在添加水平为1,3,10 mg/kg时,回收率为70.8%-93.8%,相对标准偏差（n=10）为2.4%-14%。方法可为祛痘类化妆品的日常监督提供技术支持。A method was set up for simultaneous determination of 20 prohibited substances（ lincosamides,tetracyclines,nitrofurans,chloramphenicol and metronidazole） in anti-acne cosmetics by LC-MS / MS. Samples was extracted by methanol- 1% formic acid（ 1： 1） solution,then was diluted and separated on a ZORBAX SBC18 column（ 2. 1 mm × 150 mm,3. 5 μm）. The mass spectrometer was operated by electrospray ionization and positive multiple reaction monitoring. The linearities showed good in the range of 10 - 250 ng / m L（ r 0. 995）. The limits of detection were all less than 0. 2 mg / kg. The average recoveries were 70. 8% - 93. 8%with RSDs of 2. 4% - 14% at the spiked levels of 1,3,10 mg / kg. The established method could provide technical support for the routine supervision of anti-acne cosmetics.

关 键 词：化妆品 祛痘 抗生素 液相色谱-串联质谱法 

分 类 号：O657.63[理学—分析化学]