流动注射化学发光法测定盐酸米诺环素  

Flow injection chemiluminescence method for determination of minocycline hydrochloride

作　　者：何云[1] 翟倩[1] 葛闯[1] 易钢[1] 

机构地区：[1]重庆医科大学检验医学院,临床检验诊断学教育部重点实验室,重庆400016

出　　处：《分析试验室》2014年第12期1463-1465,共3页Chinese Journal of Analysis Laboratory

摘　　要：在碱性条件下,K3[Fe（CN）6]氧化鲁米诺产生化学发光,盐酸米诺环素对该体系化学发光反应具有显著的抑制作用,据此建立了快速测定盐酸米诺环素的流动注射化学发光新方法。在优化的实验条件下,化学发光抑制强度与盐酸米诺环素的质量浓度在8.0-400 ng/m L范围内呈良好的线性关系,检出限为7.4 ng/m L。对200 ng/m L盐酸米诺环素连续平行测定11次,相对标准偏差为1.8%。方法可用于盐酸米诺环素片剂的测定。A simple,rapid and sensitive chemiluminescence method for the determination of minocycline hydrochloride was developed based on the principle that the chemiluminescence intensity of luminol-potassium ferricyanide reaction system could be inhibited by minocycline hydrochloride in alkaline condition. Under the optimum condition,the linear range for minocycline hydrochloride was 8. 0 - 400 ng / m L,and the detection limit was 7. 4 ng / m L. The relative standard deviation（ RSD） was 1. 8% for the eleven-time consecutive determinations of 0. 2 μg / m L minocycline hydrochloride. The sensitive method has been used for the determination of minocycline hydrochloride tablets.

关 键 词：化学发光 流动注射 盐酸米诺环素 鲁米诺 铁氰化钾 

分 类 号：O657.3[理学—分析化学]